香菇多糖对大鼠胶质瘤生长的影响及体外细胞抑制实验  

Inhibitory Effect of Lentinan on Glioma Growth in Vivo and in Vitro

作　　者：郑玉霞[1] 石军飞[2] 李永宏 郑志刚 田景民[4] 庞丽纹[1] 涂红[4] 

机构地区：[1]内蒙古化工职业学院,呼和浩特010070 [2]内蒙古医科大学第二附属医院,呼和浩特010030 [3]内蒙古宇航人高技术产业有限责任公司,呼和浩特010010 [4]内蒙古医科大学,呼和浩特010030

出　　处：《天然产物研究与开发》2014年第7期1108-1111,1116,共5页Natural Product Research and Development

摘　　要：为评价香菇多糖对大鼠体内胶质瘤生长的影响以及对体外胶质瘤细胞的抑制作用,本研究将大鼠建模成功后,给予生理盐水和不同剂量香菇多糖,30 d后,采用免疫组化方法观测瘤组织变化。并将胶质瘤细胞在香菇多糖处理24、48、72 h后,检测细胞活性,流式细胞术检测细胞凋亡及细胞周期。发现荷瘤大鼠在治疗30 d后,香菇多糖低、中、高剂量治疗组肿瘤的体积呈逐渐缩小趋势,与对照组相比较,香菇多糖治疗组肿瘤组织局部出现坏死灶,细胞核染色逐渐变浅,新生血管数减少。体外实验中,香菇多糖40、80 mg/L剂量组在给药72 h后,细胞凋亡率和坏死率均均明显高于对照组(P<0.01)。香菇多糖20、40、80 mg/L作用72 h后,G0/G1期细胞与对照组相比明显增加(P<0.05)。说明香菇多糖能显著抑制大鼠胶质瘤的生长,并能在体外诱导胶质瘤细胞的凋亡。The objective of this study was to investigate the impact of lentinan on the growth of glloma in rats and inhibi- tion ofglioma cell in vitro. For the animal experiment,normal saline and different doses of lentinan were gavaged to the model rats for 30d. The changes of tumor tissue were then observed by immunohistochemical methods. For the in vitro ex- periment,the glioma cells were treated by lentinan for 24,48 and 72h. The activity,apoptosis and cycle of glioma cell were then detected by flow eytometry. The experimental results showed that the tumors volume dwindled in sample group compared with the control group; Coloration of nucleus was lighter in the sample group; Number of new blood vessels was reduced. For the in vitro experiment, the apoptosis rate of glioma ceils of the 40 mg/L and 80 mg/L lentinan groups were significantly higher than that of control group （P 〈 0.01 ） after 72 h of treatment; The number of GO/G1 phase cells of the 20,40,80 mg/L lentinan groups, were increased significantly （ P 〈 0.05 ） compared with that of the control group after 72 h of treatment. These results showed that ＇the lentinan can inhibit the growth of glioma in rots, and cause the apeptosis of glioma cells in vitro.

关 键 词：香菇多糖 胶质瘤 细胞凋亡 药物作用 

分 类 号：R961.1[医药卫生—药理学]