LDH5在舌鳞状细胞癌中的表达及其临床意义  

The expression and clinical significance of LDH5 in tongue squamous cell carcinoma

作　　者：袁春平[1] 李中武[1] 张玮[2] 王东苗[1] 程杰[1] 杨建荣[1] 

机构地区：[1]南京医科大学口腔医学研究所,南京江苏210029 [2]南京医科大学附属口腔医院口腔病理科,南京江苏210029

出　　处：《南京医科大学学报（自然科学版）》2014年第7期926-929,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81100737);江苏省自然科学基金(BK2011762);教育部高等学校博士学科点专项科研基金(20113234120003)

摘　　要：目的:探讨LDH5蛋白在舌鳞状细胞癌组织中的表达特点,及其与临床病理和患者生存预后的关系。方法:应用免疫组织化学染色法检测LDH5蛋白在63例舌癌组织标本和16例正常舌黏膜组织标本中的表达。结合患者临床病理资料和随访资料,应用SPSS17.0软件分析LDH5的表达与相关临床病理参数(Fisher精确概率法)以及患者总生存率之间的关系(KaplanMeier法)。结果:LDH5在部分舌鳞癌组织中呈高表达(33/63,52.38%),与其在正常舌黏膜组织的表达水平相比有显著统计学差异(P<0.01)。LDH5蛋白表达水平与肿瘤大小、颈淋巴结转移、临床分期具有相关性(P<0.05),而与患者的性别、年龄、病理分级、局部浸润等无相关性(P>0.05)。Kaplan-Meier法分析患者总体生存情况结果显示:LDH5高表达组患者的总体生存率明显低于LDH5低表达组患者(P=0.006,Log-rank检验)。结论:LDH5在部分舌鳞状细胞癌组织中呈异常高表达,其表达水平与肿瘤的病理学特征及患者预后具有相关性。Objective：To investigate the characteristics of LDH5 expression in tongue squamous cell carcinoma（TSCC） and its relationship with clinicopathological parameters and survival. Methods：The LDH5 protein expression in 63 cases of TSCC and 16cases of normal tongue mucosa were examined by immunohistochemistry. Based on these patients＇ information and their follow-up data,the correlation of LDH5 expression with patients＇ clinicopathological characteristics and overall survival were further evaluated by SPSS17.0 software using Fisher exact test and Kaplan-Meier method,respectively. Results：The LDH5 expression in TSCC（33 /63,52.38%） was much higher than that in normal tongue（P〈0.001）. LDH5 over-expression was found to be associated with tumor size,cervical lymph node metastasis and clinical stages（P〈0.05）,but not with gender,age,local invasion and pathological grades（P〉0.05）. The overall survival rate in patients with high LDH5 expression was significantly lower than that in patients with low expression as estimated by Kaplan-Meier and Log-rank test（P = 0.006）. Conclusion：LDH5 is aberrantly over-expressed in a significant fraction of TSCC patients. Its upregulation is associated with multiple clinicopathological features and patients＇ prognosis.

关 键 词：舌鳞状细胞癌 LDH5基因 有氧糖酵解 免疫组织化学染色 

分 类 号：R739.86[医药卫生—肿瘤]