CXCR2和CXCR4对人宫颈癌HeLa细胞作用  

Effects of CXCR2 and CXCR4 on human cervical carcinoma HeLa cells

作　　者：贺雅莉[1] 黄旭[2] 花文玲[1] 黄凤凤[1] 

机构地区：[1]福建医科大学附属二院妇产科,福建泉州362000 [2]福建医科大学附属协和医院病理科

出　　处：《中国公共卫生》2014年第8期1050-1051,共2页Chinese Journal of Public Health

基　　金：福建省泉州市科技局项目(2010Z29)

摘　　要：目的了解CXCR2和CXCR4对HeLa细胞迁移、增殖和分泌VEGF的影响。方法 Transwell检测HeLa细胞迁移情况;MTT法检测CXCR2和CXCR4对HeLa细胞增殖的影响;ELISA法检测HeLa细胞VEGF表达情况。结果敲除CXCR2或CXCR4后,宫颈癌HeLa细胞迁移率分别为(2.1±0.8)和(2.6±0.9),未敲除组迁移率为(3.6±1.1),差异有统计学意义(P<0.05);激活CXCR2或CXCR4后,HeLa细胞增殖率分别为(86.1±15.3)%和(83.9±15.9)%,未激活组HeLa细胞增殖率为(66.7±8.7)%(P<0.05),激活CXCR2或CXCR4后,VEGF表达水平分别为(6.84±0.19)和(6.59±0.14)pg/L,未激活组为(0.74±0.03)pg/L(P<0.05)。结论HeLa细胞表面高表达的CXCR2和CXCR4在诱导HeLa细胞迁移、增殖和分泌VEGF方面具有重要的作用。Objective To investigate the effects of CXC chemokine receptor-2 （CXCR2） and CXC chemokine receptor-4（CXCR4） on migration, proliferation and secretion of vascular endothelial growth factor （VEGF） of HeLa cells. Methods HeLa cell migration was detected with Transwell method. Effects of CXCR2 and CXCR4 on the proliferation of the HeLa cells was detected with 3 - （ 4,5-dimethyl-2-thiazolyl ） -2,5-diphenyl-2-H-tetrazolium bromide （ MTT ） assay. The expression of VEGF of the HeLa cells was detected with enzyme-linked immunosorbent assay （ELISA）. Results After the knockout of CXCR2 and CXCR4, the mobility of the HeLa cells were 2. 1 e 0. 8 and 2.6 ± 0. 9, and the rate was 3.6 ± 1.1 for the cells without the knockout,with statistically significant differences（P 〈0.05 for all）. After the activation of CXCP,2 and CXCR4,the proliferation rates of the HeLa ceils were 86. 1 ± 15.3% and 83.9 ± 15.9% ,significantly higher than that of the cells without the activation （ 66.7 ± 8.7 % ） （ P 〈 0. 05 for all）. In addition, the VEGF expressions of the HeLa cells with the activation of CXCR2 and CXCR4 were 6. 84 ±0. 19 pg/L and 6. 59 ±0. 14 pg/L and were significantly higher than that of the cells without the activation （ 0. 74 ± 0, 03 pg/L） （ P 〈 0. 05 for all ）. Conclusion High expressions of CXCR2 and CXCR4 in HeLa cells play an important role in the induction of migration, proliferation and secretion of VEGF of HeLa cells.

关 键 词：CXCR2 CXCR4 宫颈癌 HELA细胞 

分 类 号：R737.33[医药卫生—肿瘤]