Isolation, identification and arsenic-resistance of Acidithiobacillus ferrooxidans HX3 producing schwertmannite  

作　　者：Yiqun Xu Ming Yang Ting Yao Huixin Xiong 

机构地区：[1]School of Environmental Science and Engineering, Yangzhou University

出　　处：《Journal of Environmental Sciences》2014年第7期1463-1470,共8页环境科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (Nos. 40902018, 31372133);the Jiangsu Key Laboratory of Environmental Material and Environmental Engineering (No. K13058)

摘　　要：Schwertmannite, a ubiquitous mineral present in iron oxyhydroxides formed in iron- and sulfate-rich acid media, favors incorporation of some toxic anions in its structure. We reported an iron-oxidizing bacterial strain HX3 from a municipal sludge that facilitates the formation of pure schwertmannite in cultures. Ferrous iron oxidation by the isolated strain HX3 was optimum at an initial pH of 2.0-3.3 and temperature of 28-35°C. Pure schwertmannite was found through bacterial oxidation of ferrous iron at an initial pH 2.8and temperature 28°C. Following 16 S rDNA gene sequence analysis the bacterial strain HX3 was identified as Acidithiobacillus ferrooxidans. The arsenic-resistance A. ferrooxidans HX3 showed the potential of environmental application in arsenic removal from the As（Ⅲ）- and iron-rich acid sulfate waters directly by As（Ⅲ） adsorption or the formation of schwertmannite in the environment.Schwertmannite, a ubiquitous mineral present in iron oxyhydroxides formed in iron- and sulfate-rich acid media, favors incorporation of some toxic anions in its structure. We reported an iron-oxidizing bacterial strain HX3 from a municipal sludge that facilitates the formation of pure schwertmannite in cultures. Ferrous iron oxidation by the isolated strain HX3 was optimum at an initial pH of 2.0-3.3 and temperature of 28-35°C. Pure schwertmannite was found through bacterial oxidation of ferrous iron at an initial pH 2.8and temperature 28°C. Following 16 S rDNA gene sequence analysis the bacterial strain HX3 was identified as Acidithiobacillus ferrooxidans. The arsenic-resistance A. ferrooxidans HX3 showed the potential of environmental application in arsenic removal from the As（Ⅲ）- and iron-rich acid sulfate waters directly by As（Ⅲ） adsorption or the formation of schwertmannite in the environment.

关 键 词：Isolation Identification Arsenic-resistance Acidithiobacillus ferrooxidans Schwertmannite 

分 类 号：X172[环境科学与工程—环境科学]