人牙周膜干细胞与牙周膜非干细胞差异表达microRNA靶基因及基因功能分析  

Target gene and gene function of differential expression of microRNA in human periodontal ligament stem cells and non-stem cells

作　　者：吕红兵[1] 卢兆杰[2] 郑碧琼[1] 雷丽珊[1] 卢国英[1] 傅升[3] 樊丽娜[3] 

机构地区：[1]福建医科大学附属口腔医院牙体牙髓一科,福州350002 [2]福建医科大学附属口腔医院特诊科,福州350002 [3]南京军区福州总医院口腔科,福州350002

出　　处：《中国实用口腔科杂志》2014年第7期403-406,共4页Chinese Journal of Practical Stomatology

基　　金：福建省自然科学基金面上项目(2011J01169);福建省科技厅重点项目(2011Y0024)

摘　　要：目的研究人牙周膜干细胞与牙周膜非干细胞之间差异表达的microRNA(miRNA)的靶基因并对其功能进行预测分析。方法本研究于2011年7月至2013年10月在福建医科大学附属口腔医院进行。采用基因芯片技术筛选人牙周膜干细胞与牙周膜非干细胞之间差异性表达的miRNA,并通过生物信息学对差异表达的miRNA进行靶基因预测及靶基因功能富集分析。结果与人牙周膜非干细胞相比,人牙周膜干细胞中明显上调的关键miRNA 25个,调控的关键靶基因21个;明显下调的关键miRNA 5个,调控的关键靶基因9个。结论特异性表达的miRNA主要调控的靶基因及其基因功能与人牙周膜干细胞的干性维持密切相关。Objective To study the mechanism of action and the regulatory network between the specific expression of mieroRNA （miRNA）and the target gene function in human periodontal ligament stem ceils and non-stem cells. Methods miRNA mieroarray was used to detect differential expression of miRNA between human periodontal ligament stein cells and non-stem cells. The way of biological information was used to predict the targets gene and gene function of these miRNAs, and to analyze the functional enrichment of target genes. Results Twenty-five miRNA were obviously up-regulated in human periodontal ligament stem cells and 21 key target genes were predicted. Five miRNA were obviously down-regulated in human periodontal ligament stem cells and 9 key target genes were predicted. Conclusion The tar- get genes and gene function which are regulated by specific miRNA are closely related to the sternness of human peri- odontal ligament stem cells.

关 键 词：人牙周膜干细胞 MICRORNA 干性维持 靶基因 基因功能 

分 类 号：R78[医药卫生—口腔医学]