香烟烟雾暴露对子代大鼠学习记忆能力影响的1H-MRS研究  

Effect of cigarette smoke exposure on learning and memory capacity of offspring of rats: 1H-MRS study

作　　者：蹇兆成[1] 龙金凤[1] 王鹏[1] 刘峰[1] 王锡臻[1] 孙业全[1] 王晓莉[1] 

机构地区：[1]潍坊医学院医学影像学系/附属医院影像中心,山东潍坊261053

出　　处：《中国妇幼保健》2014年第22期3660-3662,共3页Maternal and Child Health Care of China

摘　　要：目的:探讨母鼠孕期香烟烟雾暴露对子代大鼠学习记忆能力的影响及相关脑区代谢产物氢质子磁共振波谱成像(1H-MRS)的变化。方法:将受孕的母鼠暴露于香烟烟雾中直至分娩,子代大鼠养育至成鼠后,测试其与对照组子代大鼠学习记忆能力并采用1H-MRS检测子代大鼠海马区脑组织代谢产物的变化。结果:水迷宫测试吸烟组子代大鼠潜伏期时间(41.17±9.16)s明显高于对照组(19.53±3.93)s,差异具有统计学意义(P<0.01)。1H-MRS显示吸烟组子代大鼠海马区氮乙酰天冬氨酸/总肌酸(NAA/Cr)、胆碱合物/总肌酸(Cho/Cr)值(4.93±0.45,8.74±0.74)明显高于对照组(2.07±0.07,2.86±0.14),差异有统计学意义(P<0.05)。吸烟组海马区NAA/(Cho+Cr)值(0.50±0.01)较对照组(0.54±0.02)低,差异有统计学意义(P<0.05)。结论:母鼠孕期处于香烟烟雾暴露会影响子代大鼠大脑海马区的发育,从而影响其学习与记忆能力的发展。Objective： To explore the effect of cigarette smoke exposure on learning and memory capacity and related brain metabolites 1H - MRS changes of offspring rats. Methods ： The pregnant female rats were exposed to cigarette smoke until delivery, their offspring were raised to adult, the learning and memory ability and brain metabolites changes in hippocampus were measured and compared with control group. Results： The latent period in smoking group was （41.17 ± 9. 16） s, which was statistically significantly higher than that in control group （19. 53 ± 3.93 s） （P 〈 0. 01 ） ; the values of NAA/Cr and Cho/Cr in smoking group were （4. 93 ± 0. 45 ） and （8. 74 ± 0. 74）, respectively, which were statistically significantly higher than those in control group （2. 07 ± 0. 07, 2. 86 ± 0. 14 ） （ P 〈 0. 05 ） . NAA/ （Cho ＋ Cr） value in smoking group was （0.50 ±0. 01 ） , which was statistically significantly lower than that in control group （0. 54 ± 0. 02 ） （ P 〈 0.05 ） . Conclusion： Exposure to cigarette smoke during gestation can affect the development of hippocampus in pregnant rats and learning and memory ability of the offspring.

关 键 词：烟雾暴露 学习记忆能力 子代大鼠 氢质子磁共振波谱成像 

分 类 号：R742[医药卫生—神经病学与精神病学]