紫杉醇长循环热敏脂质体对Lewis肺癌荷瘤鼠的药效学研究  

Pharmacodynamics of paclitaxel long-circulating thermo sensitive liposomes in Lewis tumor-bearing mice

作　　者：李美波[1] 顾生玖[1] 赵文鹏[1] 朱开梅[1] 

机构地区：[1]桂林医学院,541004

出　　处：《实用医学杂志》2014年第14期2193-2196,共4页The Journal of Practical Medicine

基　　金：广西科技攻关项目(编号:桂科能129825-21;桂科合14123001-22);广西教育厅项目(编号:201202ZD065;2013lX080);桂林市科技攻关项目(编号:科技攻关20130103-8;20140105-11;20130103-9;20130113-1;20120105-5;20120105-16;20120105-8)

摘　　要：目的:考察紫杉醇长循环热敏脂质体(PLTL)对Lewis肺癌荷瘤小鼠的药效学。方法:建立Lewis肺癌肿瘤模型,随机分为5组,对照组、加热组、紫杉醇注射液(PTX)组、紫杉醇热敏脂质体(PTL)组和PLTL组,局部联合热疗,观察小鼠生活状态,监测肿瘤的体积及瘤重,对肿瘤组织切片进行HE染色,观察肿瘤细胞的形态学变化,采用流式细胞术检测肿瘤细胞的凋亡率。结果:PTX、PTL组和PLTL组的抑廇率分别为48.87%、57.22%和78.87%,肿瘤细胞凋亡率分别为(42.7±3.8)%,(54.6±2.9)%和(69.7±5.0)%。结论 :PLTL与PTX和PTL相比,PLTL具有明显的热敏靶向性,与局部热疗相结合显著提高了紫杉醇的治疗效果。Objective To investigate the tumor inhibition effect of paclitaxel long-circulating thermo sensitive liposomes （PLTL） in mice bearing Lewis lung carcinoma cells. Methods A tumor-bearing mouse model was established, and the mice were randomly divided into five groups： control, heating, paclitaxel （PTX）, paclitaxel thermo sensitive liposomes （PTL）, and PLTL groups. The living status was observed in the mice. The volume and weight of the tumor were measured. The morphological changes in the tumor cells were observed f by HE staining and apoptosis of the tumor cells was determined by flow cytometry. Results The inhibition rate of tumor in PTX, PTL and PLTL groups was 48.87%, 57.22%and 78.87%, respectively. The apoptotic rate of tumor cell in PTX, PTL and PLTL groups was （42.7 ± 3 .8）%, （54.6 ± 2.9）%and （69.7 ± 5.0）%, respectively. Conclusions PLTL, as compared with PTX and PTL, has an evident thermo sensitive feature and increases the anticancer effect of paclitaxel remarkably in combination with local hyperthermia.

关 键 词：紫杉醇 长循环热敏脂质体 热敏脂质体 热疗 

分 类 号：R734.2[医药卫生—肿瘤]