一种从平菇菌丝中提取总RNA的方法  

A Method for Extraction of Total RNA from Mycelium of Pleurotus ostreatus

作　　者：史红鸽 戚元成[2] 申进文[2] 

机构地区：[1]河南省驻马店市农业科学院,河南驻马店463000 [2]河南农业大学生命科学学院,河南郑州450000

出　　处：《江西农业学报》2014年第8期68-70,共3页Acta Agriculturae Jiangxi

基　　金：河南省科技攻关项目

摘　　要：本实验建立了一种从平菇菌丝中提取总RNA的方法。对利用该方法提取的总RNA进行凝胶电泳、紫外分光光度检测,结果显示:所提取RNA的条带清晰,无降解,具有较高的纯度。RT-PCR分析结果进一步表明,所提取的RNA质量较好,能够用于后续的分子生物学研究。This experiment established a method for the extraction of total RNA from the mycelium of Pleurotus ostreatus. The total RNA extracted by using this method was determined through gel electrophoresis and ultraviolet spectrometer. The results indica-ted that the RNA isolated from the mycelium of Pleurotus ostreatus showed clear bands,and possessed higher purity without degrada-tion. RT-PCR amplification results also showed that the quality of the extracted RNA was better,and could meet the demand of subsequent molecular biology studies.

关 键 词：平菇 菌丝 总RNA 提取 方法 

分 类 号：S646.14[农业科学—蔬菜学]