高效液相色谱法同时测定肉豆蔻麸煨前后肉豆蔻木脂素和去氢二异丁香酚的含量  

Determination of Content of Nutmeg Lignan and Dehydrodiisoeugenol in Nutmeg by HPLC

作　　者：刘欢[1] 陈剑锋 王静[1] 袁子民[1] 

机构地区：[1]辽宁中医药大学,大连116600 [2]辽宁成大方圆医药有限公司,沈阳110005

出　　处：《医药导报》2014年第8期1070-1072,共3页Herald of Medicine

基　　金：国家自然科学基金资助项目(81274084)

摘　　要：目的建立高效液相色谱法同时测定肉豆蔻麸煨前后肉豆蔻木脂素、去氢二异丁香酚的含量测定方法。方法色谱柱为Diamonsil C18（250 mm＆#215;4.6 mm,5μm）,流动相为甲醇-水梯度洗脱,检测波长270 nm,柱温25℃,流速1.0 mL·min-1。结果肉豆蔻木脂素在10.24-61.44μg·mL-1范围内线性关系良好,r=0.9996,平均回收率为97.94%（n=6）,RSD为2.01%。去氢二异丁香酚在3.0-18.0μg·mL-1范围内线性关系良好,r=0.9998,平均回收率为97.11%（n=6）,RSD为2.17%。结论该方法简单、准确、重复性好,可用于同时测定肉豆蔻麸煨前后肉豆蔻木脂素和去氢二异丁香酚丁香酚的含量。Objective To develope a high performance liquid chromatograph （ HPLC ） method for simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after processing of nutmeg. Methods The column was Diamonsil C18（250 mm＆#237; 4. 6 mm, 5 μm）. The mobile phase was methanol-water in a gradient elution mode. The UV detection wave length was 274 nm. The column temperature was 25℃ . The flow rate was 1. 0 mL·min-1 . Results Nutmeg lignan and dehydrodiisoeugenol had a good linear correlation in ranges of 10. 24-61. 44 μg·mL-1（r=0. 999 6） and 3. 0-18. 0 μg·mL-1 （r=0.999 8）, respectively. The average recovery rates were 97. 94% （RSD=2. 17%） and 97. 11% （RSD=2. 17%）. Conclusion The method is simple, accurate, reproducible, and can be used for the simultaneous determination of nutmeg lignan and dehydrodiisoeugenol before and after the processing of nutmeg.

关 键 词：肉豆蔻 肉豆蔻木脂素 去氢二异丁香酚 色谱法 高效液相 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]