黄芪甲苷、人参皂苷Rg_1、Rb_1和三七皂苷R_1抗小鼠脑缺血再灌注氧化应激损伤和促进能量代谢的配伍研究  

The Combination Study of Astragaloside Ⅳ, Ginsenosides Rg_1, Rb_1 and Notoginsenoside R_1 on Antagonizing Oxidative Stress Injury and Promoting Energy Metabolism after Ischemia-reperfusion in Mice

作　　者：黄小平[1] 王蓓[1] 邱咏园[1] 曾嵘[1] 邓常清[1] 唐映红[1] 

机构地区：[1]湖南中医药大学分子病理实验室,湖南省中西医结合心脑血管疾病重点实验室,细胞生物学与分子技术湖南省高校重点实验室,湖南长沙410208

出　　处：《湖南中医药大学学报》2014年第7期5-11,共7页Journal of Hunan University of Chinese Medicine

基　　金：国家自然科学基金资助项目(81102557);教育部2010年度高等学校博士学科点专项科研基金资助项目(20104323110001);湖南省高校创新平台开放基金资助项目(11K050);湖南省教育厅一般项目(11C0963);湖南省中医药管理局重点项目(201301);湖南省科技厅一般项目(2014SK3001)

摘　　要：目的从氧化应激和能量代谢研究黄芪甲苷、人参皂苷Rg1、Rb1和三七皂苷R1抗小鼠脑缺血再灌注损伤的配伍关系,明确其有效的配伍剂量。方法采用L9(34)正交试验法,将C57BL/6小鼠随机分组,连续给药3 d后结扎双侧颈总动脉造成脑缺血20 min,再灌注30 min,测定脑组织三磷酸腺苷(ATP)、还原型谷胱甘肽(Glutathione,GSH)含量、丙二醛(Malonaldehyde,MDA)的含量及超氧化物歧化酶(Superoxide Dismutase,SOD)的活性。结果黄芪甲苷、人参皂苷Rb1、Rg1和三七皂苷R1能增加脑组织中ATP、GSH含量和SOD活性,降低MDA的含量,对脑缺血再灌注后的氧化应激损伤具有抑制作用,改善脑组织能量代谢。4种有效成分配伍具有增强抗脑缺血再灌注损伤的作用。结论 4种有效成分抗小鼠脑缺血再灌注后氧化应激损伤和改善能量代谢的有效配伍剂量为黄芪甲苷40 mg/kg,人参皂苷Rg150 mg/kg,人参皂苷Rb140 mg/kg,三七皂苷R110 mg/kg。Objective To study the combination relativity among Astragaloside IV, Ginsenoside Rgl, Rbl and Notoginsenoside R1 against ischemia-reperfusion injury through oxidative stress and energy metabolism, expliciting the effective combination dose. Methods Using L9 （34） orthogonal experimental method, C57BL/6 mice were randomly grouped, treated for 3 d. At 1 h after the last administration, bilateral common carotid artery （CCA） were occluded with artery clip for 20 min followed by reperfusion for 30 min, to detect the contents of adenosine triphosphate （ATP）, glutathione （GSH）, malondialdehyde （MDA） and the activity of superoxide dismutase （SOD） in brain tissues. Results Astragaloside IV, ginsenosides Rb1, Rg1 and notoginsenoside R1 increased the contents of GSH, ATP and the activity of SOD, decreased MDA content, having inhibitory effect on oxidative stress injury after cerebral ischemia-reperfusion and improving energy metabolism of brain tissues. Four active components combination potentiated the effect against cerebral ischemia-reperfusion injury. Conclusion The effective combination dose of four active component antagonizing oxidative stress injury and improving energy metabolism after ischemia-reperfusion in mice was Astragaloside IV 40 mg/kg, ginsenosides Rgl 50 mg/kg, ginsenosides Rb1 40 mg/kg, notoginsenoside R1 10 mg/kg, respectively.

关 键 词：黄芪甲苷 人参皂苷Rg1 人参皂苷Rb1 三七皂苷R1 配伍 脑缺血再灌注 氧化应激 能量代谢 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R285.5[医药卫生—临床医学]