SIAH2蛋白在肝细胞癌发生、发展中的作用研究  

Role of SIAH2 protein in the development of hepatocellular carcinoma

作　　者：刘瑞磊[1] 王佳妮[1] 张鹏[2] 张翘楚[1] 李玺[1] 姜华[1] 黄勇[1] 

机构地区：[1]中山大学附属第三医院甲乳外科,广州510630 [2]中山大学附属第三医院岭南医院普外科

出　　处：《中华肝脏外科手术学电子杂志》2014年第3期51-54,共4页Chinese Journal of Hepatic Surgery(Electronic Edition)

基　　金：中央高校基本科研业务费专项基金(09ykpy15);广东省医学科研基金(A2011183)

摘　　要：目的探讨SIAH2蛋白在肝细胞癌(肝癌)发生、发展中的作用。方法取对数生长期的人肝癌细胞Bel-7404,分别将空载体小干扰核糖核酸(siRNA)及SIAH2 siRNA转染至人肝癌细胞Bel-7404中,将转染后细胞分为control-siRNA组和SIAH2-siRNA组。以β-肌动蛋白(β-actin)为对照,采用蛋白质印迹法检测两组人肝癌细胞Bel-7404的SIAH2蛋白表达情况。采用噻唑蓝法检测两组细胞增殖情况,采用细胞划痕实验观察两组细胞迁移情况,采用Transwell实验观察两组细胞侵袭情况。两组间的数据比较采用t检验。结果 control-siRNA组和SIAH2-siRNA组的SIAH2蛋白平均相对表达量分别为0.71±0.02、0.33±0.01。与control-siRNA组相比,SIAH2-siRNA组的SIAH2蛋白相对表达量明显减少(t=-4.629,P<0.05),增殖速度亦明显下降。control-siRNA组的细胞迁移率为(45.3±0.4)%,SIAH2-siRNA组为(14.3±0.4)%,SIAH2-siRNA组明显低于control-siRNA组(t=-3.689,P<0.05)。control-siRNA组的穿膜细胞数为(563±10)个,SIAH2-siRNA组为(122±7)个,SIAH2-siRNA组明显少于control-siRNA组(t=-3.428,P<0.05)。结论 SIAH2蛋白能促进肝癌细胞增殖、迁移、侵袭,从而加速肿瘤的发生、发展。Objective To investigate the role of SIAH2 protein in the occurrence and development of hepatocellular carcinoma （HCC）. Methods Human HCC Bel-7404 cells in logarithmic growth phase were inoculated. Empty carrier small interference ribonucleic acid （siRNA） and SIAH2 siRNA were transfected in human HCC Bel-7404 cells. Then the cells were divided into 2 groups：control-siRNA group and SIAH2-siRNA group. Theβ-actin was taken as control, and the expression of SIAH2 protein in human HCC Bel-7404 cells of 2 groups was detected by Western Blot. The proliferation of cells in 2 groups was detected by methyl thiazolyl tetrazolium （MTT） assay. Cell migration in 2 groups was observed by cell scratch test. Cell invasion in 2 groups was observed by Transwell assay. The data in 2 groups were compared using t test. Results The average relative expression of SIAN2 in control-siRNA and SIAH2-siRNA group were 0.71±0.02, 0.33±0.01 respectively. The expression of SIAN2 in SIAH2-siRNA group decreased obviously compared with that in control-siRNA group （t=-4.629, P〈0.05）. The proliferation rate in SIAH2-siRNA group also decreased obviously. The cell migration rate in SIAH2-siRNA group[（14.3±0.4）%] was significantly lower than that in control-siRNA group [（45.3±0.4）%]（ t=-3.689, P〈0.05）. The membrane permeating cell count in SIAH2-siRNA group （122±7） was signiifcantly less than that in control-siRNA group （563±10） （t=-3.428, P〈0.05）. Conclusion SIAH2 protein can promote the proliferation, migration and invasion of HCC cells and thus accelerate the occurrence and development of HCC.

关 键 词：癌 肝细胞 细胞增殖 细胞迁移分析 肿瘤侵润 转染 SIAH 

分 类 号：R735.7[医药卫生—肿瘤]