三氧化二砷与顺铂合用抗人口腔癌细胞株HSQ-89的实验研究  

Inhibition of combined application of arsenic trioxide and cisplatin in the HSQ-89 cells

作　　者：江穗[1] 叶永康[1] 陈仲伟[1] 彭参[1] 罗朝阳[1] 

机构地区：[1]广东省医学科学院、广东省人民医院口腔颌面外科 ,广州市510080

出　　处：《实用医学杂志》2014年第15期2371-2373,共3页The Journal of Practical Medicine

基　　金：广东省科技计划项目(编号:20088030301036,20078031514005);广东省中医药管理局科研项目(编号:20121260)

摘　　要：目的:探讨三氧化二砷能否增强顺铂对口腔癌细胞的抗瘤活性。方法:以人口腔癌细胞株HSQ-89为研究对象,在经顺铂处理过的肿瘤细胞中加入不同浓度的三氧化二砷,用MTT法测定计算肿瘤细胞的抑制率。结果:在加入顺铂15μg/mL的口腔癌细胞珠HSQ-89中加入不同浓度的三氧化二砷0、2.5、5、7.5、10、12.5μmol/mL,肿瘤细胞的抑制率分别为26.9%、67.5%、73.0%、88.5%、90.4%、98.7%;三氧化二砷与顺铂联用,可以提高对HSQ-89细胞的抑制率,并呈剂量依赖性增加。结论:三氧化二砷与顺铂联用,对口腔癌细胞的生长抑制具有协同效应。Objective To discuss the anticancer role of arsenic trioxide （ATO） with cisplatin on human oral carcinoma HSQ-89 cells. Methods The human oral epidermoid HSQ-89 cells were chosen as the subjects. Different concentrations of ATO were added into Cisplatin（DDP）-treated cells. The inhibition rate of tumor cells was detected by MTT assay. Results Different concentrations of ATO （0,2.5,5,7.5,10,12.5 μmol/mL） were added into oral cancer HSQ-89 cells which have been treated with DDP （15 μg/mL）. The inhibition rate of tumor cells were 26.9%, 67.5%, 73.0 %, 88.5%, 90.4%, 98.7% respectively; The combined application of ATO with cisplatin could improve the inhibition rate of HSQ-89 cells in a dose-dependent relation. Conclusion The combined application of ATO and DDP can produce a synergistic action of inhibition on oral cancer cell.

关 键 词：口腔肿瘤 三氧化二砷 顺铂 

分 类 号：R739.85[医药卫生—肿瘤]