大黄素对CT26结肠癌小鼠调节性T细胞功能的影响  

Effect of emodin on Treg cells function in a mouse model of CT26 colon cancer

作　　者：阮志燕[1] 商玲[1] 邓向亮[2] 蔡琳[1] 

机构地区：[1]广东食品药品职业学院,广州市510520 [2]广州中医药大学,510405

出　　处：《实用医学杂志》2014年第15期2377-2380,共4页The Journal of Practical Medicine

基　　金：广东省医学科学技术研究基金项目(编号:A2013145);广东省中医药局建设中医药强省立项资助科研课题(编号:20121104);广东食品药品职业学院自然科学研究项目(编号:2012YZ001)

摘　　要：目的:探讨大黄素对CT26结肠癌小鼠调节性T细胞免疫抑制功能的影响。方法:CT26结肠癌小鼠分为阴性对照组、大黄素治疗组和荷瘤模型组,给药14 d,分别检测以下指标:小鼠不同组织T细胞IFN-γ的分泌、效应细胞CD8+CD3+T细胞的比例、血清细胞因子IFN-γ、TNF-β1、IL-10的水平、不同组织CD4+CD25+Tregs IL-10的分泌。结果:大黄素明显升高CD8+CD3+T细胞在肿瘤局部的比例(P<0.05);大黄素显著提高外周血和淋巴结中T细胞分泌IFN-γ的能力(P<0.05);大黄素显著降低IFN-γ、TNF-β1、IL-10在血清中的水平(P<0.01);大黄素有抑制CD4+CD25+Tregs分泌IL-10的作用(P<0.01)。结论:大黄素可能通过影响Tregs的免疫抑制功能发挥抗肿瘤的作用。Objective To investigate the effect of emodin on immune suppression function of regulatory T cells in a mouse model of CT26 colon cancer. Methods Twenty-four mice were divided into the negative control group, the emodin group and the tumor group. The populations of CD8＋CD3＋T cells, the T cells producing IFN-γ/ and the CD4＋CD25＋/Tregs secreting IL-10 in different mouse tissues were detected by flow cytometry. Levels of IFN-γ, TNF- β1 and IL-10 in serum were determined by ELISA. Results Emodin could significantly increase the percent of CD8＋CD3＋ cells in tumor （P 〈 0.05） and improve the ability of IFN-γ, secretion in T cells from peripheral blood and lymph nodes （P 〈 0.05）. Emodin could reduce the levels of IFN-γ, TNF-β1 and IL-10 in the serum （P 〈 0.01） and inhibit IL-10 secretion in CD4＋CD25＋ Tregs （P 〈 0.01）. Conclusion Emodin possesses the antitumor effect by affecting the immunosuppressive function of Tregs cells.

关 键 词：结肠肿瘤 大黄素 CT26结肠癌 调节性T细胞 

分 类 号：R735.35[医药卫生—肿瘤]