蜡梅亲免素基因CpFKBP的克隆与表达分析  

On Cloning and Characterizing CpFKBP Gene fromChimonanthus Praecox(L.)

作　　者：王斐[1] 秦朝[1] 刘祥[1] 眭顺照[1] 李名扬[1] 

机构地区：[1]西南大学园艺园林学院,重庆市花卉工程技术研究中心,南方山地园艺学教育部重点实验室,重庆400716

出　　处：《西南师范大学学报（自然科学版）》2014年第4期48-53,共6页Journal of Southwest China Normal University(Natural Science Edition)

基　　金：国家自然科学基金项目(31070622和30872063)基金资助

摘　　要：在已构建好的蜡梅花cDNA文库及EST分析的基础上,随机克隆测序得到一个蜡梅FKBP基因,将其命名为CpFKBP,Genebank登录号:JQ337962.该基因cDNA长为482bp,具有一个339bp的ORF,编码一个113个氨基酸的多肽,预测等电点为8.48,理论分子量为12.08KD.在大肠杆菌中获得并纯化了重组的CpFKBP蛋白质,并进一步对重组蛋白进行PPIase活性分析,结果显示CpFKBP重组蛋白有较高的PPIase酶活性.A FKBP gene has been obtained by randomly sequencing the cDNA library of Chimonanthus praecox flowers,which is named as CpFKBP (GenBank accession No:JQ337962).The 482 bp cDNA of CpFKBP contains a 339 bp open reading frame (ORF)encoding a 113-amino-acid polypeptide with a cal-culated molecular mass of 12.08 KD and the theoretical pI of 8.48.The recombinant CpFKBP with the fused TRX and His Tags at it N-terminus has been purified by the His-binding purification Kit.The analy-sis of PPIase activity shows that the fusion protein of CpFKBP has a high PPIase activity.This study has facilitated the research of the FKBP genes in different species,the application of immuno-suppressants and understanding the whole immunophilin family.

关 键 词：蜡梅 FKBPs 原核表达 PPIASE 

分 类 号：Q785[生物学—分子生物学] S685.99[农业科学—观赏园艺]