CD40信号联合细胞因子体外制备过继免疫治疗细胞的实验研究  

Experimental study on in vitro preparation of adoptive immunotherapy cells induced by CD40 in combination with cytokines

作　　者：高腾飞[1] 古彦铮[2] 徐俊驰 沈宇[2] 李晓晨[1] 朱一蓓[1] 张学光[2] 

机构地区：[1]苏州大学医学部基础医学与生物科学学院,苏州215013 [2]苏州大学附属第一医院,苏州215006 [3]苏州第五人民医院,苏州215007

出　　处：《中国免疫学杂志》2014年第7期933-936,941,共5页Chinese Journal of Immunology

基　　金：江苏省自然科学基金面上项目(BK2011289)资助

摘　　要：目的:探索一种新的过继免疫治疗细胞体外培养的方法。方法:从正常人外周血分离单个核细胞(PBMCs),经过CD40激发型单抗5C11、细胞因子IFN-α、IL-7、IL-2(CD40激发组)诱导并扩增,光镜下观察其细胞形态及计数,进行动态增殖观察,并在第9天收集细胞,对其淋巴细胞亚群、NKT细胞和调节性T细胞(Treg)的比例进行分析,同时与常规CIK细胞(CD3激发组)进行比较性分析。结果:两组培养细胞中CD4+/CD8+细胞的比例无明显差异;但CD3激发组中的Treg数量和比例远高于CD40激发组,而CD3+CD56+的NKT细胞低于CD40激发组,同时,CD40激发组出现了一群单核-NK-DC(Mo-NKDC)样细胞。结论:本实验所建立的新型过继免疫治疗细胞培养方法能提高具有肿瘤杀伤能力的NKT细胞的比例,同时显著减少Treg的数量,有望为肿瘤细胞过继免疫治疗开拓新的方案。Objective：To explore a new method of the cultivation of adoptive immunotherapy cells.Methods： Mononuclear cells was isoplated by density gradient centrifugation and then proliferated by using CD 40-agonist monoclonal antibody 5C11、cytokine of IFN-αand IL-7（CD40 group）in vitro.During the culturing procedure ,the cell morphology was obersved by optical microscope.The percentage of T-lymphocytes, NK-T cells, Treg cells and the cell proliferation, which were compared with CIK group CD3mAb activated,was detected on the 9th day.Results：There was no significant difference of CD4^＋/CD8^＋T cells percentage between the two groups.But the Treg cells percentage of CIK group was far higher than that of CD 40 group,while the percentage of CD3^＋CD56^＋NK-T cells was lower than that of CD 40 group.And a group of Mo-NK-DC cells were observed in the CD 40 group.Conclusion： The new method of adoptive immunity therapy has been established in this study could increase the percentage of NK -T cells which had the ability to kill tumor cells.Simultaneously ,it is reduced the amount of Treg cells significantly.

关 键 词：过继免疫治疗细胞 CD40激发型单抗5C11 IFN-Α IL-7 

分 类 号：R73[医药卫生—肿瘤]