4种海星的α-葡萄糖苷酶抑制活性研究  

Inhibitory activity of α-glucosidase in four starfishes

作　　者：张改云[1] 范林[2] 侯忠海[2] 张玉便[1] 杨艳柳 

机构地区：[1]国家海洋局第三海洋研究所生物遗传资源重点实验室,福建厦门361005 [2]东华大学化学化工与生物工程学院,上海201620

出　　处：《药学实践杂志》2014年第4期284-287,共4页Journal of Pharmaceutical Practice

基　　金：福建省科技计划农业科技重点项目(2011N0019)

摘　　要：目的寻找具有降糖活性作用的海星并确定其活性部位。方法首先采用正常和糖尿病小鼠口服糖耐量试验,比较4种海星粗提物改善口服糖耐量的作用,然后利用体外α-葡萄糖苷酶抑制剂模型进一步确定具有改善口服糖耐量作用的海星中抑制α-葡萄糖苷酶的活性部位。结果罗氏海盘车粗提物对正常小鼠和糖尿病小鼠餐后0.5、1 h血糖均有显著降低作用,其中的正丁醇部位具有很强的α-葡萄糖苷酶抑制活性。结论罗氏海盘车的正丁醇部位具有α-葡萄糖苷酶抑制作用。Objective To search for starfishes with hopoglycemic activity and orientate the bioactive fractions.Methods Firstly,the crude extracts of four starfishes were subjected to oral glucose tolerance test on normal and diabetic mice.And then the in vitro α-glucosidase activity assay was introduced to confirm the bioactive fractions.Results The crude extract of Asterias rollestoni could lower the blood glucose of normal and diabetic mice after 0.5 h and 1 h.Furthermore,its n-BuOH fraction exhibited potent α-glucosidase inhibitoary activity.Conclusion The n-BuOH extract of Asterias rollestoni had a potent α-glucosidase inhibitoary activity.

关 键 词：海星 罗氏海盘车 糖耐量 Α-葡萄糖苷酶 四氧嘧啶 

分 类 号：R282.74[医药卫生—中药学]