PEBP4、CR-1在食管癌中表达的临床意义  

Clinical Significance of PEBP4,Cripto-1 Expressions in Esophageal Carcinoma

作　　者：罗东[1] 尹杰[1] 柯有力[1] 

机构地区：[1]湖北省武汉市第一医院心胸外科,武汉市430022

出　　处：《广西医学》2014年第9期1205-1208,共4页Guangxi Medical Journal

摘　　要：目的探讨磷脂酰乙醇胺结合蛋白4(PEBP4)、畸胎瘤衍化生长因子(CR-1)在食管癌组织中表达的临床意义。方法选取食管癌组织(癌组织组)及正常食管黏膜组织(正常组)各30份,分别采用免疫组化法、蛋白质印迹法检测各组PEBP4、CR-1表达水平。结果癌组织组PEBP4表达阳性率为83.3%,显著高于正常组的20.0%(P<0.05);有淋巴结转移及远处转移的癌灶PEBP4表达阳性率显著高于无淋巴结转移及无远处转移癌灶(P<0.05)。癌组织组CR-1表达阳性率为86.7%,显著高于正常组的16.7%(P<0.05);有淋巴结转移及远处转移的癌灶CR-1表达阳性率显著高于无淋巴结转移及无远处转移癌灶(P<0.05)。癌组织组CR-1 mRNA及其蛋白相对表达水平均显著高于正常组(P<0.05)。结论食管癌组织中PEBP4、CR-1阳性表达率较高,对食管癌的早期诊断及预后判断有较高临床价值。Objective To explore the clinical significance of expressions of phosphatidylethanolamine binding protein 4（ PEBP4）,teratocarcinoma derived growth factor（ Cripto-1,CR-1） in esophageal carcinoma tissues. Methods Thirty cases of esophageal cancer tissues and 30 case of normal esophageal mucosa tissues were selected,respectively.The expressions of PEBP4,CR-1 were detected by immunohistochemical technique and Western Blot,respectively.Results The positive rate of PEBP4 expression in the esophageal cancer group was 83. 3%,significantly higher than that in the normal group（ 20. 0%）. The positive rate of PEBP4 expression in the lesion with lymph node metastasis and distant metastasis tumor was significantly higher than that without metastasis（ P〈0. 05）. The positive rate of CR-1expression in the esophageal cancer group was 86.7%,significantly higher than that in the normal group（ 16.7%,P〈0. 05）.The positive rate of CR-1 expression in the lesion with lymph node metastasis and distant metastasis tumor was significantly higher than that without metastasis（ P〈0. 05）. The relative expressions of CR-1 mRNA and protein in the esophageal cancer group were significantly higher than those in the normal group（ P〈0. 05）. Conclusion PEBP4 and CR-1 in esophageal cancer tissues have a higher positive expression rate,which is valuable in the early diagnosis and prognosis of esophageal carcinoma.

关 键 词：食管癌 磷脂酰乙醇胺结合蛋白4 畸胎瘤衍化生长因子 免疫组化法 蛋白质印迹法 

分 类 号：R735.1[医药卫生—肿瘤]