敲除基因iha对尿路致病性大肠杆菌生物被膜形成的影响  

Effects of iha Gene Knockout on Uropathogenic Escherichia coli Biofilm Formation

作　　者：谢中斌 王利霞[1] 

机构地区：[1]陕西省榆林市第二医院检验科,榆林市719000

出　　处：《广西医学》2014年第9期1209-1211,共3页Guangxi Medical Journal

摘　　要：目的探讨敲除基因iha对尿路致病性大肠杆菌生物被膜的影响。方法通过Red重组系统的pcP20、pkD3及pkD46质粒将菌株W140中的iha基因敲除,将噬菌体中的3个重组蛋白转入W140菌株使其具有相同的重组能力,氯霉素抗性基因替换敲除的iha基因,最后获得敲除iha基因的菌株,比较野生菌株与敲除iha基因的菌株生物被膜形成能力的差异。结果野生W140菌株扩增出了2 091 bp的iha基因,而阳性克隆子扩增出了1 133 bp的氯霉素抗性基因片段,但无iha基因片段;此外,iha敲除菌株的生物膜形成能力显著低于野生菌株(P<0.05)。结论 iha基因在尿路致病性大肠杆菌感染中有重要作用,敲除iha基因可以抑制生物被膜的形成,从而控制感染。Objective To study the effects of iha gene knockout on uropathogenic Escherichia coli biofilm.Methods iha gene was knocked out in W140 by using the recombination system pcP20,pkD3 and pkD46 plasmids.Three recombinant proteins were transfected into W140 to gain the same restructuring ability. Chloramphenicol resistance gene was used to replace iha gene. The bacteria with iha being knocked out were acquired. The differences of the ability to form biofilm were compared between wild and iha knockout strains. Results The wild W140 strain with 2 091 bp was amplified. The positive clone of chloramphenicol resistance gene with 1 133 bp was amplified,while it had no iha gene segment. In addition,the ability of biofilm formation of iha knockout strain was significantly weaker than that of wild strain（ P〈0. 05）. Conclusion iha gene plays an important role in the uropathogenic Escherichia coli infection. iha gene knockout can inhibit the formation of biofilm so as to control the infection.

关 键 词：大肠杆菌 iha基因 细菌生物被膜 尿路感染 基因敲除 

分 类 号：R574.6[医药卫生—消化系统]