茶黄素在大鼠气道上皮细胞炎症损伤中的作用及可能机制  

Effect of theaflavin on LPS-induced inflammatory injury of rat airway epithelial cell and possible mechanism

作　　者：文秀芳[1] 陈霞[1] 邬海桥[1] 周向东[2] 

机构地区：[1]重庆市第三人民医院呼吸内科,400014 [2]重庆医科大学第二临床学院呼吸内科,400010

出　　处：《重庆医学》2014年第21期2756-2758,共3页Chongqing medicine

基　　金：重庆市科委资助项目(cstc2013jcyjA1062)

摘　　要：目的研究茶黄素对原代培养的大鼠气道上皮细胞表达TLR4及分泌TNF-α和IL-6的影响。方法用脂多糖(LPS)建立体外大鼠气道上皮细胞炎症损伤模型,采用ELISA检测气道上皮细胞分泌TNF-α、IL-6和气道上皮细胞中TLR4蛋白水平,RT-PCR检测气道上皮细胞TLR4mRNA的水平。结果 LPS明显诱导大鼠气道上皮细胞分泌TNF-α、IL-6,同时增强TLR4mRNA和TLR4蛋白的表达(P<0.01)。茶黄素可抑制LPS诱导TNF-α、IL-6的分泌和TLR4基因的表达(P<0.05),并且与茶黄素药物浓度有关(P<0.05)。结论在大鼠的气道上皮细胞培养中,茶黄素抗炎作用可能通过抑制TLR4基因的表达、LPS/TLR4信号传导通路,从而减少炎性细胞因子的释放。Objective To study the influence of theaflavin on the expressions of TLR4 and release of TNF-αand IL-6 in primari-ly cultured rat airway epithelial cells .Methods Lipopolysacchride（LPS） was adopted to establish the in vitro inflammatory injury model of rat airway epithelial cell ,the TNF-αand IL-6 secreted by the airway epithelial cells and the TLR4 protein level in the air-way epithelial cells were detected with ELISA and the expressions of TLR4 mRNA was detected with RT-PCR .Results LPS sig-nificantly induced the airway epithelial cells to secrete TNF-αand IL-6 ,and enhanced the TLR4 mRNA and TLR4 protein expres-sion（P〈0 .01） .Theaflavin could inhibit LPS induced TNF-αand IL-6 secretion and TLR4 gene expression（P〈0 .05） ,which was related with the theaflavin concentration （P〈0 .05） .Conclusion In rat airway epithelial cell culture ,the anti-inflammatory effect of theaflavin may be decrease the release of inflammatory cytokines via inhibiting TLR 4 gene expression and LPS/TLR4 signal trans-duction pathway .

关 键 词：肿瘤坏死因子Α 白细胞介素-6 脂多糖 茶黄素 TLR4 mRNA 

分 类 号：R56[医药卫生—呼吸系统]