去甲斑蝥素联合ABT-737对宫颈癌细胞增殖及凋亡的影响  

Inducing Apoptosis of Norcantharidin in Combination with ABT-737 on Cervical Cancer Cells

作　　者：王娜[1] 张菊新[1] 刘广芝[1] 

机构地区：[1]郑州大学附属人民医院妇产科,450003

出　　处：《国际生殖健康／计划生育杂志》2014年第4期261-264,共4页Journal of International Reproductive Health/Family Planning

基　　金：国家自然科学基金(39870854)

摘　　要：目的:研究去甲斑蝥素(norcantharidin,NCTD)联合ABT-737对宫颈癌细胞增殖及凋亡的影响。方法:采用四甲基偶氮唑盐(MTT)法检测NCTD单药或联合ABT-737对宫颈癌细胞株HeLa、SiHa增殖的影响;采用流式细胞术分析NCTD联合ABT-737对宫颈癌细胞凋亡的影响。结果:NCTD单药能够显著抑制宫颈癌细胞株HeLa、SiHa的增殖,抑制作用呈剂量依赖性。NCTD与ABT-737联用后抑制作用明显增强。1μmol/L ABT、60μmol/L NCTD及两药联用诱导HeLa细胞的凋亡率分别为(31.65±7.75)%、(40.34±14.52)%和(63.03±10.90)%,诱导SiHa细胞的凋亡率分别为(9.76±0.04)%、(22.04±3.03)%和(58.36±2.31)%。NCTD、ABT两药联合的促凋亡作用比单药显著增强(P<0.05)。结论:NCTD单药在体外能够明显抑制宫颈癌细胞HeLa、SiHa的增殖,与ABT-737联用后抑制增殖和促凋亡作用更加显著。Objective：Norcantharidin （NCTD） is a new small molecule with anticarcinogen activity, which was extracted from Chinese cantharides. ABT-737 is another small molecule with antiearcinogen activity in lower dose by inhibiting BCL-2. This study was designed to explore the effect of NCTD combined with ABT- 737 on the in vitro proliferation and apoptosis of cervical cancer cells. Methods：HeLa and SiHa cell lines were cultured in vitro. NCTD alone or in combination with ABT-737 was used to treat cells. MTT assay was used to determine the viability of ceils. The flow cytometry was used to detect apoptosis. Results：NCTD alone could significantly inhibit the proliferation of HeLa and SiHa in a dose-dependent manner. NCTD combined with ABT-737 could significantly enhance the effect of NCTD. The apoptosis rates of HeLa in the ABT-737 （1 μmol/L） group and the NCTD （60 μmol/L） group were （31.65±7.75）% and （40.34±14.52）%, while this rate was （63.03±10.90）% in the NCTD combined ABT-737 group. The apoptosis rates of SiHa in the ABT-737 group and the NCTD group were （9.76±0.04）% and （22.04±3.03）%, respectively; while the rate in the NCTD combined ABT-737 group was （58.36±2.31）%. Effect of the combined two drugs on apoptosis was significantly stronger than that of any single drug （P〈0.05）. Conclusions：NCTD shows the in vitro anti-proliferation effect on the HeLa and SiHa cell lines and the effects of both inhibiting proliferation and inducing apoptosis when combined with ABT-737.

关 键 词：宫颈肿瘤 癌 斑蝥素 细胞增殖 细胞凋亡 

分 类 号：R737.33[医药卫生—肿瘤]