16S rRNA测序鉴定1株条件致病性人苍白杆菌  

Identifying 1 strain of conditional pathogenic Ochrobactrumby 16S rRNA gene sequencing

作　　者：洪文艳[1] 李曦[1] 刘建伟[1] 于德宪[1] 谢晓波[1] 刘金华[1] 唐博恒[1] 

机构地区：[1]广州军区疾病预防控制中心疾病监控科,广东广州510507

出　　处：《国际检验医学杂志》2014年第14期1819-1820,共2页International Journal of Laboratory Medicine

基　　金："艾滋病和病毒性肝炎等重大传染病防治"科技重大专项传染病监测技术平台项目(2009-ZX10004-205)

摘　　要：目的探索一种基于16SrRNA基因的细菌快速鉴定方法,为临床未知病原菌的诊断及治疗提供科学依据。方法对临床患者的痰标本分离培养纯菌落,直接以菌液为模板,以通用引物PCR扩增未知菌的16SrRNA基因片段,产物直接测序。将测序结果进行BLAST比对,根据序列同源性鉴定病原细菌。结果未知病原菌经本实验鉴定为人苍白杆菌,经ABI细菌快速鉴定板条检测,确认结果一致。结论该研究简化了临床标本未知病原菌分离培养鉴定的步骤,建立了一种利用16SrRNA基因扩增快速鉴定病原菌的简便方法。Objective To explore a rapid bacterial identifying method based on the 16S rRNA gene sequence analysis technology to provide the scientific basis for the diagnosis and treatment of unknown pathogenic bacteria.Methods The pure colonies were iso-lated and cultured directly from a clinical patient′s sputum sample.The colony as a template for PCR amplification with universal primers to amplify 16S rRNA gene fragments of unknown bacteria.The product of PCR was sequenced directly,then the sequence result was compared by using the BLAST of NCBI and the pathogen was identified based on the sequence homology.Results 1 strain of unknown pathogen was identified as ochrobactrum by this test and confirmed by ABI bacterial rapid identification sys-tem.Conclusion This study simplifies the isolation and identification procedures of unknown pathogen from the clinical samples and establishes a simple method for the rapid identification of pathogens by using 16S rRNA gene amplification.

关 键 词：病原检测 细菌鉴定 直接PCR 16S RRNA 

分 类 号：R446.5[医药卫生—诊断学]