免疫细胞化学染色在乳腺癌诊断中的应用价值及质量控制  

Application value and quality control of immunocytochemical staining in diagnosis of breast cancer

作　　者：贺才标 吴涛 朱娅萍[1] 叶红[3] 胡志勇[3] 王林[3] 陈勇 魏贝[3] 

机构地区：[1]湖北省天门市妇幼保健院检验科,湖北天门431700 [2]湖北省天门市中医院放射科,湖北天门431700 [3]湖北省天门市第一人民医院病理科,湖北天门431700

出　　处：《国际检验医学杂志》2014年第14期1923-1924,1927,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨乳腺癌患者癌细胞ER、PR、C-erbB-2、P53、Ki67表达的应用价值及质量控制。方法采用细针吸取细胞学涂片或术中细胞学印片获取标本,联合免疫细胞化学染色,研究53例乳腺癌患者癌细胞ER、PR、C-erbB-2、P53、Ki67的生物学表达,并与免疫组织化学染色进行对照分析。结果 53例乳腺癌患者ER、PR、C-erbB-2、P53、Ki67的生物学表达分别为73.58%、58.49%、56.60%、68.63%、81.13%,ER、PR、C-erbB-2、P53、Ki67阳性病例和阳性强度,免疫细胞化学染色与免疫组织化学染色之间差异均无统计学意义(P>0.05)。结论免疫细胞化学染色可替代免疫组织化学染色,在乳腺癌的术前诊断、治疗方案的选择、以及预后的判断具有重要的意义。全过程的质量控制是做好乳腺癌免疫细胞化学染色的关键。Objective To investigate the application value and quality control of breast cancer cells PR,ER C-erbB-2,P53,Ki67 expressions in the patients with breast cancer.Methods The specimens were obtained by the fine needle aspiration cytological smear or intraoperative imprint cytology.The biological expressions of ER,PR,C-erbB-2,P53,Ki67 were detected by the immuno-cytochemical staining,the detection results with those detected by the immunohistochemical staining were performed the compara-tive analysis.Results The biological expressions of ER,PR,C-erbB-2,P53,Ki67 in 53 cases of breast cancer were 73.56%, 58.49%,56.60%,68.63%,81.13% respectively;ER,PR,C-erbB-2,P53,Ki67 had no statistical significant differences in positive cases and positive intensity between the immunocytochemical staining and the immunohistochemical staining （P 〉0.05）.Conclusion The immunocytochemical staining can replace the immunohistochemical staining and has the important significance in the preop-erative diagnosis of breast cancer,therapeutical scheme selection and prognosis judgment.The whole process quality control is the key to do the breast cancer immunocytochemical staining well.

关 键 词：乳腺癌 免疫细胞化学技术 质量控制 

分 类 号：R737.9[医药卫生—肿瘤]