埃克替尼通过p38-MAPK信号通路对涎腺腺样囊性癌ACC-M细胞的凋亡诱导作用  

Induction effect of icotinib on apoptosis of salivary adenoid cystic carcinoma ACC-M cells through p38-MAPK pathway

作　　者：杨彩玲[1] 张景航[2] 张应花[3] 任铭新[3] 刘进忠[4] 崔卫刚[3] 

机构地区：[1]新乡医学院第一附属医院口腔外科,河南新乡453100 [2]新乡医学院病理科,河南新乡453003 [3]新乡医学院人体解剖学教研室,河南新乡453003 [4]郑州大学口腔医学院口腔病理教研室,河南郑州450052

出　　处：《吉林大学学报（医学版）》2014年第4期820-823,共4页Journal of Jilin University：Medicine Edition

基　　金：河南省教育厅科学技术研究重点项目资助课题(14B320018)

摘　　要：目的:探讨埃克替尼对人涎腺腺样囊性癌细胞ACC-M凋亡的影响,阐明埃克替尼对涎腺腺样囊性癌的治疗作用机制。方法:ACC-M细胞随机分为对照组,2、4、8μmo1·L-1埃克替尼组,促分裂原活化蛋白激酶(APK)抑制剂SB203580(20μmol·L-1)组,SB203580(20μmol·L-1)+埃克替尼(4μmol·L-1)组。4h后收集细胞,采用MTT法检测各组ACC-M细胞的生长抑制率,用caspase-3活力检测试剂盒检测ACC-M细胞的凋亡情况(即caspase-3活力),采用Western blotting法检测p-p38-MAPK蛋白的表达水平。结果:与对照组比较,埃克替尼组ACC-M细胞生长抑制率明显升高(P<0.05),caspase-3活力显著增强(P<0.05),p-p38-MAPK蛋白表达水平增加(P<0.05)。与4μmol·L-1埃克替尼组比较,SB203580+埃克替尼组p-p38-MAPK蛋白表达水平明显降低(P<0.05),caspase-3活力显著降低(P<0.05)。结论:埃克替尼可通过上调p-p38-MAPK信号的表达诱导ACC-M细胞凋亡。Objective To explore the influence of icotinib in the apoptosis of the human salivary adenoid cystic carcinoma cells ACC-M,and to clarify the mechanism of icotinib for the treatment of salivary adenoid cystic carcinoma.Methods The ACC-M cells were randomly divided into control group,2,4,8μmo1·L^-1 icotinib groups,p38-MAPK inhibitor SB203580（20μmol·L^-1）group,SB203580（20μmol·L^-1）＋4μmo1·L^-1icotinib group;the cells were collected 4hafter treatment.The viability of ACC-M cells was measured by MTT assay.The apoptosis of ACC-M cells was assessed by caspase-3activity kit.The expression of p-p38-MAPK protein was determined by Western blotting analysis.Results Compared with control group,the inhibitory rates of growth of the ACC-M cells in icotinib groups were significantly decreased（P〈0.05）,and the activities of caspase-3were increased（P〈0.05）,and the expression levels of p-p38-MAPK were significantly increased（P〈0.05）.Compared with 4μmo1·L^-1 icotinib group,the expression level of p-p38-MAPK in SB203580＋icotinib group were decreased（P 0.05）,and the activity of caspase-3 was decreased dramatically（P 〈0.05）.Conclusion Icotinib may induce the apoptosis of ACC-M cells through the activation of p38-MAPK signaling pathway.

关 键 词：埃克替尼 涎腺腺样囊性癌细胞 促分裂原活化蛋白激酶 细胞凋亡 

分 类 号：R739.86[医药卫生—肿瘤]