P53和ras癌基因表达产物联合检测在胃癌早期诊断中的意义  

Combining-detection expression of p53 and ras gene in gastric cancer and Its clinical significance

作　　者：冯卫华[1] 冯玉荣[1] 刘定军[1] 

机构地区：[1]河南省焦作市人民医院内二科,河南焦作454150

出　　处：《河北医学》2002年第5期394-396,共3页Hebei Medicine

摘　　要：目的 :为了研究p5 3和ras癌基因表达产物 (p5 3蛋白和ras蛋白 )在胃癌早期诊断中价值。方法 :对 2 2例浅性胃炎 ,2 5例重度不典型增生 ,30例早期胃癌标本进行p5 3和p2 1蛋白免疫组化检测。结果 :在浅性胃炎中两种蛋白均为阴性 ;重度不典型增生和早期胃癌中两种蛋白阳性率非常接近 ,其差异不显著 (P >0 .0 5 ) ,但均高于浅表性胃炎 ,差异性显著 ,(P <0 .0 5 )p5 3和p2 1蛋白联合检测阳性率 ,在重度不典型增生 (76 .0 % )和早期胃癌 (70 .0 % )中 ,均高于两组中p5 3蛋白单项阳性率 (40 .0 %和 36 .7% ) ,和两组中p5 3和p2 1蛋白同时阳性率 (2 4 .0 %和 2 6 .7% ) ,其差异性均显著 (P <0 .0 5 )。结论 :重度不典型增生在p5 3和ras基因表达水平上接近早期胃癌 ,二种基因表达产物联合检测在胃癌早期诊断中有临床应用价值。Objective:To investigate the relationship between the overexpression of p21.@ras, p21protein and P53 protein,and the value to the early diagnosis of the gastric cancer.Method:30 cases of early gastric cancer,and 25 cases of severe dysplasia were selcted as the objecets of study,and 22 cases of super fieial gastritis were taken as control in this study.Results:There was significant difference between gastritis and severe dysplasia and early gastric cancers(P<0.05),but there were significant difference between severe dysplasia and early gastric cancer(P>0.05).Combining-detection positiveness was 76.0% in the severe dysplasia,70.0% in the early gastric cancer,all significanly higher than the positive rates:40.0% and 36.7% of the p53 protein,and 24.0% and 26.7% of the pogsitiveness of both p53 and p21 protein in the severe dysplasia and early gastric cancer (P<0.05).Conclusions:The positive expressions of p53 and ras gene were similar between those in the severe dysplasias and early gastric cancers.The clinical value of p53 and p21protien combining-detection was higher in the early diagnosis of gastric cancer.

关 键 词：P53基因表达 RAS基因表达 胃癌 免疫组化 诊断 

分 类 号：R735.2[医药卫生—肿瘤]