亚砷酸联合顺铂抑制鼻咽癌细胞和逆转RASSF1A甲基化状态的作用  

Effects of arsenic trioxide combined with cisplatin on the growth of human nasopharyngeal carcinoma cells and reversion of RASSF1A hympermethylation

作　　者：黄雪琴[1,2] 王晓刚[1] 胡俊丽[1] 周慧[1] 张月飞[1] 

机构地区：[1]广东医学院附属医院耳鼻咽喉-头颈外科,广东湛江524001 [2]广东医学院第二临床学院耳鼻咽喉科,广东东莞523808

出　　处：《临床耳鼻咽喉头颈外科杂志》2014年第14期1061-1065,共5页Journal of Clinical Otorhinolaryngology Head And Neck Surgery

基　　金：广东省科技计划项目;湛江市科技攻关计划项目(No:2011C3109010);广东医学院面上培育项目(No:XK1449)

摘　　要：目的:研究亚砷酸(As2O3)及联合顺铂(DDP)作用于人鼻咽癌裸鼠移植瘤后对RASSF1A表达的影响。方法:建立人鼻咽癌CNE2细胞裸鼠移植瘤模型,随机分为NaCl组、As2O3组、DDP组和As2O3加DDP联合组,对标本分别采用RT-PCR法检测RASSF1AmRNA的表达;免疫组织化学SP法检测RASSF1A蛋白的表达;HRM法检测RASSF1A启动子区甲基化率。结果:各实验组均能抑制人鼻咽癌CNE2裸鼠移植瘤生长,并能明显上调抑癌基因RASSF1A的表达,各实验组RASSF1A的甲基化率明显低于NaCl组,且As2O3与DDP联合优于As2O3、DDP单一用药。结论:As2O3抑制人鼻咽癌CNE2裸鼠移植瘤的生长,促进RASSF1A的表达。As2O3可抑制鼻咽癌细胞恶性表型和逆转RASSF1A的甲基化状态。ObjectiveTo investigate the effect of arsenic trioxide （AszO3） combined with cisplatin on expres-sion of RASSF1A in nude mice with human nasopharyngeal carcinoma xenograft. Method.. The models of humanpoorly differentiated nasopharyngeal carcinoma in nude mice were established and randomly divided into fourgroups, control group（NaC1 group）, As2O3 group, DDP group and As2O3-FDDP group. The expression ofRASSF1A mRNA and protein were detected by Real-time RT-PCR and immunohistochemistry respectively. Themethylation rate of RASSF1A promoter CpG islands was analyzed by HRM. Results： Experimental groups couldobviously inhibit the growth of tumor and up-regulate the expression of RASSF1A. The methylation rate ofRASSF1A in transplanted tumors in experimental groups was lower than the control group. Especially As2O3combined with DDP were superior to the single drug use. Conclusion.. As2O3 inhibits the growth of human naso-pharyngeal carcinoma cell strain CNE2 xenograft in nude mice and increases mRNA expression of RASSF1A.As2O3 inhibits the malignant phenotypes of human nasopharyngeal carcinoma cells and reverses hypermethylationof RASSF1A.

关 键 词：亚砷酸 鼻咽肿瘤 RASSF1A 甲基化 

分 类 号：R739.63[医药卫生—肿瘤]