小细胞肺癌患者血清抗纺锤体抗体检测的临床意义  

The clinical significance of human mitotic spindle apparatus antibody detection in the serum of patients with small cell lung cancer

作　　者：明凤[1] 谭立明[1] 陈娟娟[1] 李华[1] 蔡励利[1] 王外梅[1] 

机构地区：[1]南昌大学第二附属医院检验科

出　　处：《免疫学杂志》2014年第7期628-631,共4页Immunological Journal

基　　金：江西省卫生厅科研计划支持项目(20133075)

摘　　要：目的探讨小细胞肺癌(SCLC)患者血清抗纺锤体抗体(MSA)检测的临床意义,了解MSA与SCLC的相关性。方法用间接免疫荧光法(IIF)和酶联免疫吸附法(ELISA)分别检测2011年9月至2012年12月南昌大学第二附属医院门诊及住院确诊的60例SCLC、164例其他肿瘤患者及40例健康对照的MSA,并对结果进行回顾性分析。结果 IIF法和ELISA法检测SCLC患者MSA阳性率分别31.7%和43.3%,ELISA法检测其MSA质量浓度为(92.3±65.2)pg/ml,均高于其他肿瘤组及对照组(P<0.01);IIF法和ELISA法对SCLC组MSA定性检测一致性分析Kappa值为0.474,对SCLC诊断的临床评价指标比较,ELISA法的敏感性为43.3%、阳性似然比为17.3%,均高于IIF法。结论 SCLC患者检测MSA抗体,对SCLC患者的诊断、鉴别诊断及治疗提供了一种全新的科学方法,可提高SCLC诊疗水平。The study aimed to detect the level of mitotic spindle apparatus antibody （MSA） in patients with small cell lung cancer （SCLC）, and to explore the correlation between MSA and SCLC. In the study, we tested serum MSA levels by indirect immunoassay fluorescence （IIF） and enzyme linked immunosorbent assay （ELISA） in 60 cases with SCLC, 164 cases with other cancer, and 40 healthy donors as control from the Second Affiliated Hospital of Nanehang University between September 2011 and December 2012. The results were retrospectively analyzed and the data showed that the positive rate of MSA in SCLC group was 31.7% and 43.3% by IIF and ELISA, respectively; the concentration of MSA in SCLC group was （92.3±65.2） pg/ml, which significantly higher than that in other cancer groups or healthy control group （P〈 0.01）. In the qualitative consistency analysis of IIF and ELISA, the kappa value for MSA was 0.474 in patients with SCLC. For clinical diagnosis comparison, the sensitivity and positive likelihood ratio of ELISA was respectively 43.3% and 17.3%, higher than that of IIF. In conclusion, this study shows serum MSA detection may be helpful for the diagnosis and differential diagnosis of SCLC.

关 键 词：小细胞肺癌 肺鳞癌 肺腺癌 抗纺锤体抗体 

分 类 号：R734.2[医药卫生—肿瘤]