Influence of casein phosphopeptide-amorphous calcium phosphate application,smear layer removal, and storage time on resin-dentin bonding  

酪蛋白磷酸肽-无定形磷酸钙、不同玷污层的处理和储存时间对牙本质粘接的影响(英文)

作　　者：Jun LIN Wei-ying ZHENG Peng-ruo-feng LIU Ning ZHANG Hui-ping LIN Yi-jing FAN Xin-hua GU Oliver VOLLRATH Christian MEHL 

机构地区：[1]Department of Stomatology, the First Affiliated Hospital, School of Medicine, Zhejiang University [2]Institute of Medical Informatics and Statistics, University Hospital Schleswig-Holstein,Campus Kiel, 24105 Kiel, Germany [3]Department of Prosthodontics, Propaedeutics and Dental Materials, Christian-Albrechts University at Kiel,Arnold-Heller-Straβe 16,24105 Kiel, Germany

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2014年第7期649-660,共12页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：Project supported by the National Natural Science Foundation of China(Nos.81271955 and 30973350);the Zhejiang Provincial Natural Science Foundation of China(No.Y2080338)

摘　　要：The aim of this study is to evaluate the influence of Tooth Mousse （TM） application, smear layer removal, and storage time on resin-dentin microtensile bond strength （pTBS）. Dentin specimens were divided into two groups： （1） smear layer covered; （2） smear layer removed using 15% EDTA for 90 s. In each group, half the specimens were treated once with TM for 60 min. After bonding procedures using a two-step self-etching adhesive （Clearfil SE Bond （CSE）; Kuraray Medical, Tokyo, Japan）, an all-in-one adhesive （G-Bond （GB）; GC Corp, Tokyo, Japan）, and a total-etch adhesive （Adper Single Bond 2 （SB）; 3M ESPE, St. Paul, MN, USA）, the specimens were stored for 3 d or 6 months in deionized water at 37 ℃, and pTBS was tested and analyzed. With the exception of SB （no TM application） and GB, the pTBS was significantly increased for CSE and SB using EDTA pre-conditioning and 3 d of storage （P≤0.001）. Bond strength of GB decreased significantly when using EDTA （3 d storage, P〈0.05）. TM application only increased the pTBS of GB （no EDTA） and SB （with EDTA） after 3 d （P≤0.02）. Comparing the adhesives after 3 d of storage, CSE exhibited the greatest pTBS values followed by GB and SB （P≤0.02）. The factors of adhesive, EDTA, and TM did not show any significant impact on pTBS when specimens were stored for 6 months （P〉0.05）. The additional application of TM and EDTA for cavity preparation seems only to have a short-term effect, and no influence on pTBS of dentin bonds after a period of 6 months.研究目的:评价含有酪蛋白磷酸肽-无定形磷酸钙(CPP-ACP)的再矿化剂Tooth Mousse(TM)的使用、不同玷污层的处理以及样本储存时间对牙本质粘接微拉伸性能的影响。研究方法:将牙本质样本分成保留玷污层组和用15%乙二胺四乙酸(EDTA)处理90秒去除玷污层组。每组根据是否使用TM处理再分亚组。每个亚组分别用三种不同的粘结剂(两步法自酸蚀Clearfil SE Bond(CSE)、一步法自酸蚀G-Bond(GB)和全酸蚀Adper Single Bond 2(SB))与牙本质样本粘接,分别经过3天和6个月的去离子水储存。样本进行切割微拉伸测试并通过扫描电镜分析断裂界面模式。重要结论:经过再矿化剂TM预处理,可以减少牙齿的敏感性,并且对于这三种粘结系统经过长时间储存后的粘结性能没有影响。EDTA的处理对于长期储存的粘结性能没有显著影响。额外的TM和EDTA对短期(3天)粘接力会有效应,但对长期(6个月)的粘接力没有影响。

关 键 词：Tooth Mousse Ethylenediaminetetraacetic acid （EDTA） Casein phosphopeptide-amorphous calcium phosphate Smear layer Bond strength 

分 类 号：R783.1[医药卫生—口腔医学]