Sirt1在神经胶质瘤及瘤旁组织中的表达差异研究  

The different expression of Sirt1 in the tissues of human glioma and para-glioma.

作　　者：陈旭[1] 张锦前[2] 刘藏[1] 王月平[1] 侯瑞光[1] 

机构地区：[1]首都医科大学附属北京友谊医院神经外科,北京100050 [2]首都医科大学附属北京地坛医院传染病研究所,北京100015

出　　处：《临床和实验医学杂志》2014年第16期1332-1335,共4页Journal of Clinical and Experimental Medicine

摘　　要：目的研究神经胶质瘤及瘤旁组织中的沉默信息调节因子1(Sirt1)基因的表达差异。方法应用基因芯片技术检测30例Sirt1基因在神经胶质瘤及瘤旁组织的表达差异,随后应用RT-PCR及免疫组织化学实验验证并分析Sirt1基因的表达差异。结果基因芯片筛选出存在差异表达的基因,其中Sirt1基因在神经胶质瘤中的表达较瘤旁组织中的表达明显下降,RT-PCR实验证实与基因芯片结果一致,免疫组织化学实验结果与基因芯片、RT-PCR结果一致。结论Sirt1在神经胶质瘤及瘤旁组织中的表达存在明显差异,Sirt1基因的表达可能与神经胶质瘤的发生发展密切相关。Objective To explore the changes in gene Sirtl expressions in tissues of human glioma and para - glioma. Methods A 44 000 gene cDNA microarray （Affymetfix） was used to examine gene expressions in the tissues of human glioma and para - glioma. The Sirtl genes were identified and subjected to real - time PCR analysis. Results The expression of Sirtl in tissues of glioma was extremely lower than that in tissues of para - glioma. The results of real - time PCR were consistent with those of genechip analysis. Conclusion The expressions of Sirtl were obviously different between tissues of glioma and para - glioma. Sirtl may be associated with carcinogenesis of glioma.

关 键 词：神经胶质瘤 沉默信息调节因子1(Sirt1) 实时荧光定量PCR 免疫组织化学 

分 类 号：R739.4[医药卫生—肿瘤]