口腔鳞状细胞癌中 STING 的表达及意义  

Expression of STING in oral squamous cell carcinoma and its clinical significances

作　　者：陈盛[1,2,1,3] 泥艳红[1,2,1,3,4] 丁亮 黄晓峰[1,2,1,3] 

机构地区：[1]南京大学口腔医学院 [2]南京市口腔医院口腔病理科,南京210008 [3]南京市口腔医院中心实验室,南京210008 [4]江苏省医学分子技术重点实验室,南京210093

出　　处：《临床与实验病理学杂志》2014年第7期744-746,750,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：国家自然科学基金(81072213、81271698);国家临床重点专科建设项目(2011);江苏省临床医学科技专项项目(BL2012017);南京市医学重点实验室建设项目(2012);南京市医学科技发展项目(YKK09124、YKK13145);江苏省卫生厅面上科研课题(H201344);南京卫生青年人才(QRX113311)

摘　　要：目的探讨干扰素刺激基因(stimulator of interferon genes,STING)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)组织及其对应癌旁组织中的表达及其临床诊断意义。方法收集25例OSCC组织及对应癌旁组织,提取组织内mRNA,采用SYBR Green荧光实时定量PCR技术检测组织中STING、TBK-1和IRF-3的表达,并分析正常角朊细胞系和OSCC细胞系中IRF-3的表达。结果 OSCC组织内STING的表达与对应癌旁组织相比显著下降(P=0.014 1);OSCC组织内STING下游基因TBK-1的表达与对应癌旁组织相比显著下降(P=0.030 6);OSCC组织内IRF-3的表达与对应癌旁组织相比显著下降(P=0.0313);OSCC细胞系HSC-3与正常细胞系HaCaT细胞系相比,IRF-3 mRNA表达显著降低(P=0.009 3)。结论 STING及其下游基因在OSCC癌灶组织内低表达,下调的STING-TBK-1-IRF-3通路可能与癌症进程有关。Purpose This study focused on the expression of STING （ stimulator of interferon genes） and its clinical diagnostic value by comparing tissues from normal and primary oral squamous cell carcinoma （OSCC） specimens. Methods The OSCC tissue （ n = 25 ） were collected and their mRNA were abstracted. The relative expression of STING, TBK-1 and IRF-3 were quantified by SYBR Green real-time PCR. Results There was significant decrease mRNA expression of STING and TBK-1 between tumor tissues and pre-carcinoma tissues of OSCC patients （P = 0. 014 1 and P = 0. 030 6）. Moreover, we discovered the mean relative expression of IRF-3 mRNA from tumor specimens were lower than pre-carcinoma tissues, so did the cell line （ P = 0. 009 3 ）. Condusion The pathway of STING-TBK-I-IRF-3 is down-regulated in OSCC and may participated in the progression of oral-related carcinoma.

关 键 词：口腔肿瘤 鳞状细胞癌 干扰素刺激基因 干扰素 

分 类 号：R739.8[医药卫生—肿瘤]