微卫星vWA和D5S818与精神分裂症的多态性分析  

Relationship between polymorphism of microsatellite vWA and D5S818 with schizophrenia

作　　者：杨光[1] 魏巍[1] 刘辉[1] 

机构地区：[1]大连医科大学检验医学院,辽宁大连116044

出　　处：《大连医科大学学报》2014年第3期234-236,共3页Journal of Dalian Medical University

摘　　要：目的探讨微卫星vWA和D5S818的多态性与精神分裂症有关的易感基因区域。方法对57例精神分裂症患者(精神分裂症组)和123名随机人群(对照组)采用PE Profiler plus系统进行PCR复合扩增,然后用ABI公司的310型基因分析仪自动进行电泳分离和基因检测。结果精神分裂症组的vWA-14的检出率为21.9%,对照组检出率为33.3%,两者之间差异有显著性意义(P<0.05);D5S818位点的各等位基因频率的检出率两组之间差异无显著性意义(P>0.05)。经吻合度检验,2个微卫星各等位基因频率的分布,均符合Hardy-Weinberg平衡。结论 vWA-14位点与精神分裂症有明显关联,提示vWA-14可能与精神分裂症某种抵抗基因连锁,表明在第12号染色体上存在与精神分裂症相关的基因。Objective To explore a genetic relationship between the polymorphism of microsatellites vWA and D5S818 with schizophrenia. Methods DNA samples collected from patients with schizophrenia （ n = 57 ） and random population （ n = 123） were amplified by PE Profiler plus system for multiplex PCR. Then ABI 3130 -Avent genetic analyzer was used for automatic capillary electrophoresis and genotyping. Results The detection ratios of vWA - 14 were 21.9 % and 33.3 % in schizophrenia group and control group, respectively. The difference was statistically significant between the two groups （P 〈0.05）. While there was no significant difference in the frequency of D5S818 between the groups （P 〉0.05）. No deviations from the Hardy - Weinberg equilibrium were observed. Conclusion There is a strong association of vWA - 14 with schizophrenia, suggesting a possible linkage between VWA - 14 and some schizophrenia resistant genes. There may be genes on chromosome 12 associated with susceptibility to schizophrenia.

关 键 词：微卫星DNA 精神分裂症 第12号染色体 

分 类 号：R749.3[医药卫生—神经病学与精神病学]