Drosophila heparan sulfate 3-0 sulfotransferase B Null Mutant Is Viable and Exhibits No Defects in Notch Signaling  

作　　者：Yueqin Guo Ying Feng Zhouhua Li Xinhua Lin 

机构地区：[1]State Key Laboratory of Biomembrane and Membrane Biotechnology,Institute of Zoology,Chinese Academy of Sciences [2]University of Chinese Academy of Sciences [3]School of Optometry and Ophthalmology and Eye Hospital,Wenzhou Medical University [4]State Key Laboratory of Stress Cell Biology,School of Life Sciences,Xiamen University [5]School of Life Sciences,Capital Normal University [6]Division of Developmental Biology,Cincinnati Children's Hospital Medical Center

出　　处：《Journal of Genetics and Genomics》2014年第7期369-378,共10页遗传学报（英文版）

基　　金：supported by the grants from the National Basic Research Program of China(Nos.2011CB943901,2011CB943902 and 2011CB943802);the National Natural Science Foundation of China(Nos.31030049,31271582 and 31071284);Strategic Priority Research Program of the Chinese Academy of Sciences Grant(No.XDA01010101)

摘　　要：Heparan sulfate proteoglycans （HSPGs） are critically involved in a variety of biological events. The functions of HSPGs are determined by the nature of the core proteins and modifications of heparan sulfate （HS） glycosaminoglycan （GAG） chains. The distinct O-sulfo- transferases are important for nonrandom modifications at specific positions. Two HS 3-0 sulfotransferase （Hs3st） genes, Hs3st-A and Hs3st-B, were identified in Drosophila. Previous experiments using RNA interference （RNAi） suggested that Hs3st-B was required for Notch signaling. Here, we generated a null mutant of Hs3st-B via ends-out gene targeting and examined its role（s） in development. We found that homozygous Hs3st-B mutants have no neurogenic defects or alterations in the expression of Notch signaling target gene. Thus, our results strongly argue against an essential role for Hs3st-B in Notch signaling. Moreover, we have generated two independent Hs3st-A RNAi lines which worked to deplete Hs3st-A. Importantly, Hs3st-A RNAi combined with Hs3st-B mutant flies did not alter the expression of Notch signaling components, arguing that both Hs3st-A and Hs3st-B were not essential for Notch signaling. The establishment of Hs3st-B mutant and effective Hs3st-A RNAi lines provides essential tools for further studies of the physiological roles of Hs3st-A and Hs3st-B in development and homeostasis.Heparan sulfate proteoglycans （HSPGs） are critically involved in a variety of biological events. The functions of HSPGs are determined by the nature of the core proteins and modifications of heparan sulfate （HS） glycosaminoglycan （GAG） chains. The distinct O-sulfo- transferases are important for nonrandom modifications at specific positions. Two HS 3-0 sulfotransferase （Hs3st） genes, Hs3st-A and Hs3st-B, were identified in Drosophila. Previous experiments using RNA interference （RNAi） suggested that Hs3st-B was required for Notch signaling. Here, we generated a null mutant of Hs3st-B via ends-out gene targeting and examined its role（s） in development. We found that homozygous Hs3st-B mutants have no neurogenic defects or alterations in the expression of Notch signaling target gene. Thus, our results strongly argue against an essential role for Hs3st-B in Notch signaling. Moreover, we have generated two independent Hs3st-A RNAi lines which worked to deplete Hs3st-A. Importantly, Hs3st-A RNAi combined with Hs3st-B mutant flies did not alter the expression of Notch signaling components, arguing that both Hs3st-A and Hs3st-B were not essential for Notch signaling. The establishment of Hs3st-B mutant and effective Hs3st-A RNAi lines provides essential tools for further studies of the physiological roles of Hs3st-A and Hs3st-B in development and homeostasis.

关 键 词：DROSOPHILA heparan sulfate proteoglycans Hs3st-A Hs3st-B NOTCH 

分 类 号：Q78[生物学—分子生物学]