IL-6对卵巢癌细胞bcl-2、cyclinD1和VEGF表达的影响  

Effects of IL-6 on the expressions of bcl-2,cyclinD1 and VEGF in ovarian cancer cells

作　　者：仇会会[1] 叶丽平[1] 温有锋[2] 李丹[1] 宋佳[1] 

机构地区：[1]辽宁医学院基础医学院病理生理学教研室,辽宁锦州121000 [2]辽宁医学院基础医学院解剖学教研室,辽宁锦州121000

出　　处：《山东大学学报（医学版）》2014年第6期17-21,共5页Journal of Shandong University:Health Sciences

基　　金：辽宁省博士科研启动基金项目(20101065);辽宁省教育厅高等学校科研项目(2009A469)

摘　　要：目的探讨白介素6(IL-6)对卵巢癌SKOV3细胞株中B-细胞淋巴瘤2(Bcl-2)、细胞周期蛋白D1(CyclinD1)和血管内皮生长因子(VEGF)的影响。方法不同浓度IL-6作用于细胞,找出IL-6最佳作用浓度。在IL-6最佳浓度基础上,加不同浓度的AG490。MTT、流式细胞术、RT-PCR、Western blotting检测细胞增殖、凋亡及细胞Bcl-2、CyclinD1、VEGF mRNA和蛋白表达。结果 IL-6(0、10、20 ng/mL)对细胞的作用呈浓度依赖性,促进细胞增殖,抑制其凋亡(P<0.01);Bcl-2、CyclinD1、VEGF蛋白和mRNA表达增加(P<0.01),48 h与24 h差异有统计学意义(P<0.01)。AG490(0、20、40、80μmol/L)对细胞的作用呈浓度依赖性,抑制细胞增殖,促进其凋亡(P<0.01);Bcl-2、CyclinD1、VEGF蛋白和mRNA表达下调(P<0.01),48 h与24 h差异无统计学意义(P>0.05)。结论 IL-6通过调节STAT3通路,调节细胞Bcl-2、CyclinD1和VEGF的表达,从而促进卵巢癌细胞的增殖,抑制其凋亡。Objective To investigate the effects of interleukin-6 （IL-6） on the expressions of B-cell lymphoma-2 （Bcl-2）, CyclinD1 （ CyclinD1 ） and vascular endothelial growth factor （ VEGF ） in epithelial ovarian cancer SKOV3 cells. Methods The optimal concentration of IL-6 was determined by treating SKOV3 cells with IL-6 of diverse concentra-tions.In addition to IL-6 of the optimal concentration, AG490 of diverse concentrations were also used.The prolifera-tion and apoptosis of cells, and the mRNA and protein expressions of Bcl-2, CyclinD1, and VEGF were detected by MTT, flow cytometry, RT-PCR and Western blotting.Results IL-6 promoted proliferation and inhibited apoptosis of the cells（P0.05）.Conclusion IL-6 promotes the gene transcription and expressions of Bcl-2, CyclinD1 and VEGF by pro-moting the phosphorylation of STAT3, and then enhances the proliferation and inhibits apoptosis of ovarian cancer cells.

关 键 词：白介素-6 卵巢癌 B-细胞淋巴瘤2 细胞周期蛋白D1 血管内皮生长因子 

分 类 号：R737.3[医药卫生—肿瘤]