大黄素对骨肉瘤细胞增殖的抑制及相关机制  

Inhibitory effect and its mechanism of emodin on the proliferation of human osteosarcoma cell line

作　　者：徐良志 陈昆仑[2] 

机构地区：[1]核工业四一七医院骨科,陕西临潼710600 [2]西安交通大学医学院第二附属医院普外科,陕西西安710004

出　　处：《山东大学学报（医学版）》2014年第6期37-40,45,共5页Journal of Shandong University:Health Sciences

摘　　要：目的探讨大黄素对人骨肉瘤细胞增殖的影响及其相关机制。方法培养人骨肉瘤细胞系SOSP9607,分为对照组及不同浓度大黄素组(25、50和100μmol/L)。MTT法检测各组细胞生长抑制率(IR);流式细胞术分析各组细胞凋亡情况;Western blotting法检测各组细胞中caspase-3、caspase-9、ERK1/2和p-ERK1/2蛋白表达水平的改变。结果 MTT法检测结果显示,与对照组相比,不同浓度大黄素对骨肉瘤细胞均有抑制作用,呈现明显的量-效关系;流式细胞技术检测结果显示,与对照组相比,大黄素能够明显促进人骨肉瘤细胞SOSP9607的凋亡,细胞凋亡率随其浓度增加而增加。Western blotting法检测结果显示,与对照组相比,不同浓度大黄素组caspase-3和caspase-9蛋白表达明显增加,呈明显的量-效关系。而p-ERK1/2蛋白表达量随着大黄素浓度的增加而减少。结论大黄素能抑制骨肉瘤细胞增殖,其机制可能是通过抑制ERK通路的活化,进一步促进caspase-3和-9蛋白的表达与活化,最终导致肿瘤细胞凋亡。Objective To explore the inhibitory effect of emodin on the proliferation of human osteosarcoma cells SOSP9607 and the related mechanisms.Methods Human osteosarcoma cells SOSP9607 were divided randomly into control group and treatment groups （emodin：25, 50 and 100μmol/L）.The growth inhibition ratio （IR） of emodin on osteosarcoma cells was detetced with MTT assay.The apoptosis was analyzed with flow cytometry.The changes of expressions of caspase-3, caspase-9, ERK1/2 and p-ERK1/2 were tested with Western blotting.Results All of the cells in the treatment groups were obviously inhibited compared to those in control group, which was dose-dependent. Compared with control group, the apoptosis ratio of treatment group increased.Western blotting showed that the expres-sions of caspase-3 and caspase-9 protein increased in treatment group compared to control group, while the expression of p-ERK1/2 protein decreased.Conclusion Emodin could inhibit the proliferation of human osteosarcoma cell by sup-pressing ERK signaling pathway and activating caspase-3 and caspase-9.

关 键 词：骨肉瘤 大黄素 细胞凋亡 ERK1 2 

分 类 号：R738.1[医药卫生—肿瘤]