TGF-β1和α-SMA在人多囊肾发病中的表达  

Expressions of TGF-β1 and α-SMA in autosomal dominant polycystic kidney disease

作　　者：阙新祥[1] 丁森泰[1] 吴斐[1] 毕东滨[1] 吕家驹[1] 丁克家[1] 

机构地区：[1]山东大学附属省立医院泌尿外科,山东济南250021

出　　处：《山东大学学报（医学版）》2014年第7期78-81,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金(ZR2011HQ027)

摘　　要：目的探讨转化生长因子β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)在常染色体显性遗传性多囊肾病(ADPKD)中的表达,及其在发病机制中的作用。方法选取62例ADPKD患者的肾组织,按照术前患肾的肾小球滤过率(GFR)将患者分为高滤过率组(A组)29例及低滤过率组(B组)33例,选取20例行肾癌根治性切除术患者的癌旁肾组织作为对照组。釆用免疫组织化学染色法分别检测TGF-β1和α-SMA在3组中的表达差异。结果 A、B两组在肾间质纤维化区域可见TGF-β1及α-SMA阳性表达,呈索状及斑块状分布;对照组无明显表达,TGF-β1及α-SMA与对照组比较差异均具有统计学意义(P<0.05)。α-SMA在A、B两组间的阳性表达率差异有统计学意义(P<0.05)。TGF-β1、α-SMA表达呈正相关(r=0.898,P<0.001)。结论 ADPKD肾组织高表达TGF-β1和α-SMA,两者相互促进上皮间质转化,导致肾组织纤维化。Objective To investigate the expressions and mechanism of transforming growth factor-β1（ TGF-β1） and alpha smooth muscle actin（ α-SMA） in autosomal dominant polycystic kidney disease（ ADPKD）. Methods According to the preoperative glomerular filtration rate（ GFR） of abnormal kidney,62 ADPKD patients were divided into two groups： high GFR group（ group A,n = 29）,and low GFR group（ group B,n = 33）. Another 20 patients treated with radical nephrectomy were chosen as controls（ the control group）. Kidney tissues from patients in groups A and B,and tumor-adjacent tissues from the controls were collected. Expressions of TGF-β1 and α-SMA of the three groups were detected with immunohistochemical method. Results TGF-β1 and α-SMA were positively expressed in groups A and B,with cord-like or patch-like distribution in the renal interstitial fibrosis area. No significant expression was found in the control group. The positive expression rate of α-SMA in groups A and B was statistically significant（ P〈 0. 05）.The expressions of TGF-β1 and α-SMA were positively correlated（ P 〉0. 05）. Conclusion TGF-β1 and α-SMA are highly expressed in ADPKD,both of which promote epithelial-to-mesenchymal transition,leading to renal fibrosis.

关 键 词：常染色体显性遗传性多囊肾病 肾纤维化 转化生长因子Β Α-平滑肌肌动蛋白 上皮间质转化 

分 类 号：R692[医药卫生—泌尿科学]