β肾上腺素受体和血管内皮生长因子受体在婴幼儿血管瘤组织中的表达  

The expression and significance of β2-AR and VEGFR-2 in infantile hemangioma

作　　者：徐广琪[1] 牛静静[1] 吕仁荣[1] 周生儒[1] 霍然[1] 

机构地区：[1]山东大学附属省立医院烧伤整形美容外科,济南250021

出　　处：《中华整形外科杂志》2014年第4期274-278,共5页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金（81171828）

摘　　要：目的 检测不同时期婴幼儿血管瘤组织中β肾上腺素受体-2和血管内皮生长因子受体-2的表达,并探讨两者在血管瘤的病理演变过程中的意义.方法 按照Mulliken分类法将血管瘤分为三期：增生期（＜12个月）32例,消退期（13 ～ 60个月）17例和消退完成期（61个月至12岁）11例;另收集7例瘤旁正常皮肤作为对照.通过免疫组化法检测增生期、消退期、消退完成期婴幼儿血管瘤组织中β肾上腺素受体-2和血管内皮生长因子受体-2的表达情况,并利用计算机图像分析技术（Image Pro Plus 6.0软件）测量平均吸光度值.数据统计以均数±标准差表示,采用SPSS16.0软件对定量结果进行单因素方差分析.结果 β肾上腺素受体-2在增生期血管瘤呈强阳性表达、消退期呈阳性表达、消退完成期呈弱阳性表达,在各期平均吸光度值分别为0.064 751 2±0.012 747、0.031 601 7±0.006 848、0.011 869 8±0.039 349;血管内皮生长因子受体-2在血管瘤增生期呈强阳性表达、消退期呈阳性表达、消退完成期呈弱阳性表达,在各期平均吸光度值分别为0.068 940 9±0.029 274、0.028 445 5±0.006 396、0.011 184 1±0.004 198;各期之间差异有统计学意义（P ＜0.05）;β肾上腺素受体-2与血管内皮细胞生长因子受体-2平均吸光度值的相关性分析具有统计学意义（P＜0.05）.结论 β肾上腺素受体-2和血管内皮生长因子受体-2可能参与了血管瘤发生、发展及消退的病理过程.Objective To investigate the significance of β-adrenergic receptor 2 （β2-AR） and vascular endothelial growth factor-2 （VEGFR-2） in the occurrence and development of infantile hemangioma through detecting the expression of β2-AR and VEGFR-2 in the different stages of infantile hemangiomas.Methods According to the Mulliken＇s classification standard,we classified the specimens as proliferating group （32 cases）,involuting group （17 cases） and involuted group （11 cases）.Normal skin tissue surrounding the hemangioma from 7 cases were chosen as control group.The expression of β2-AR and VEGFR-2 was detected by immunohistochemical technique in proliferating hemangioma,involuting hemangioma,involuted hemangioma.The mean optical density was measured by image analysis system （Image Pro Plus 6.0） and SPSS 16.0 software was applied for statistical analysis.Results The expression of β2-AR and VEGFR-2 was strongly positive in proliferating hemangioma,while positive in involuting hemangioma and weakly positive in the involuted stage.The mean optical density of each phase was 0.064 751 2 ±0.012 747,0.031 601 7 ±0.006 848,0.011 869 8 ±0.039 349 for β2-AR,and 0.068 940 9±0.029 274,0.028 445 5 ±0.006 396,0.011 184 1 ±0.004 198 for VEGFR-2.The differences between different stages had a statistically significance（P ＜ 0.05）.Correlation analysis on the mean optical density between β2-AR and VEGFR-2 had a statistically significance （P ＜ 0.05）.Conclusions β2-AR and VEGFR-2 may be involved in the occurrence and development of infantile hemangioma.

关 键 词：婴幼儿血管瘤 β肾上腺素受体-2 血管内皮生长因子受体-2 

分 类 号：R732.2[医药卫生—肿瘤]