应用滚环扩增技术检测沙眼衣原体L型  

Detection of Chlamydia Trachomatis Serovars L1,L2,L3 Using Rolling Circle Amplification

作　　者：王辉[1] 孔繁荣[1] 周小勇[1] 王玮蓁[1] 段逸群[1] 

机构地区：[1]湖北省感染性皮肤病研究中心,湖北省武汉市第一医院皮肤科,湖北武汉430022

出　　处：《中国皮肤性病学杂志》2014年第8期861-863,共3页The Chinese Journal of Dermatovenereology

摘　　要：目的建立滚环扩增技术检测沙眼衣原体L型(L1,L2,L3)的方法。方法使用沙眼衣原体Omp1靶基因。设计衣原体L1,L2,L3型特异性锁式探针。使用衣原体Omp基因通用引物扩增标本,L1,L2,L3型特异性锁式探针与扩增的靶基因杂交结合形成环化单链分子,加入引物在Corbett RotorGeneTM 6000扩增仪滚动扩增环化单链分子,观察结果。结果滚环扩增技术检测上述3型衣原体,各型之间无交叉反应。结论滚环扩增作为一种新的扩增技术能检测L型衣原体。Objective To develop a novel padlock probe and rolling circle amplification （RCA） to detect Chlamydia trachomatis serovars L1, L2, L3. Methods Primers （CTSb and CTAb） were used to amplify the Chlamydia trachomatis Ompl gene. Amplicon was ligate mollicute specific padlock probe. Probe amplified and monitored using a Corbett RotorGeneTM 6000 machine. Results Chlamydia trachomatis serovars L1, L2, L3 were correctly detected by RCA method. There was no cross-reaction. Conclusion Rolling circle amplification was a promised method for detection and identification of Chlamydia trachomatis serovars L1, L2, L3.

关 键 词：滚环扩增 锁式探针 衣原体L型 

分 类 号：R574.1[医药卫生—消化系统]