D-乳酸合成阻断对肺炎克雷伯氏菌的1,3-丙二醇发酵的影响  

Influence of blocking formation of D-lactic acid on 1,3-propanediol fermentation by Klebsiella pneumoniae

作　　者：周佳佳[1] 高黎荣[1] 刘龙飞[1] 张书翠[1] 付水林[1] 宫衡[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《工业微生物》2014年第4期24-27,共4页Industrial Microbiology

摘　　要：利用Red同源重组技术,快速敲除肺炎克雷伯氏菌中的编码D-乳酸脱氢酶的两个基因——ldhA和dld,获得KG1-1和KG1-2两个突变株,并研究了敲除编码D-乳酸脱氢酶基因丧失合成D-乳酸的KG1-1菌株的1,3-PD产量和菌体生长变化,实验结果表明乳酸合成缺失对现有工艺1,3-丙二醇发酵无影响。The impact of blocking D-lactate formation by direct knocking out the genes of D-lactic dehydrogenase in Klebsiella pneumoniae was investigated. The two genes of ldhA and dld were knocked out separately to construct two mutants of KG1-1 and KG1-2. The results showed that KGI-1 was a D-lactate deficient mutant, while its 1,3-PD production and cell growth were not influenced on the present technology.

关 键 词：肺炎克雷伯氏菌 D-乳酸脱氢酶 1 3-丙二醇 RED同源重组 

分 类 号：TQ923[轻工技术与工程—发酵工程]