PSGL-1^(+/-);IL-2^(-/-)小鼠模型的构建  

The construction of PSGL-1^(+/-);IL-2^(-/-) mice model

作　　者：亓翠玲[1] 叶杰[1] 杨扬[1] 李斌[1] 何晓东[1] 李江超[1] 章倩倩[1] 黄韧 王丽京[1] 

机构地区：[1]广东药学院血管生物学研究所,广东广州510006 [2]广东省实验动物检测所,广东广州510663

出　　处：《中国现代医生》2014年第23期7-9,共3页China Modern Doctor

基　　金：国家自然科学基金(31271455)

摘　　要：目的构建PSGL-1+/-;IL-2-/-小鼠模型。方法将白介素2敲除(IL-2 KO)小鼠与P-选择素糖蛋白配体-1敲除(PSGL-1 KO)小鼠杂交,剪尾提DNA后用PCR法检测小鼠的基因型。结果 IL-2 KO小鼠与PSGL-1 KO小鼠杂交三代后,PSGL-1+/-;IL-2-/-小鼠模型构建成功。结论将IL-2 KO小鼠与PSGL-1 KO小鼠杂交三代后,成功建立了PSGL-1+/-;IL-2-/-小鼠模型。Objective To construct the PSGL-1＋/-;IL-2-/- mice model. Methods PSGL-1 KO mice were mated with IL- 2 KO mice. Mice were genotyped by PCR after cut the tail and extracted DNA. Results After PSGL-1 KO mice being mated with IL-2 KO mice for three generations,PSGL-1＋/-;IL-2-/- mice were obtained. Conclusion PSGL-1＋/-;IL-2-/- mice are established through PSGL-1 KO mice being mated with IL-2 KO mice.

关 键 词：P-选择素糖蛋白配体-1 白介素-2 P-选择素糖蛋白配体-1敲除小鼠 白介素-2敲除小鼠 动物模型 

分 类 号：R-332[医药卫生]