补阳还五汤对失神经肌萎缩大鼠Angptl4表达的影响  

Effect of Buyang Huanwu Decoction on Angptl4 expression in denervated tibial muscle atrophy of rats

作　　者：周岚[1] 梅晓云[1] 

机构地区：[1]南京中医药大学基础医学院,江苏南京210023

出　　处：《中成药》2014年第8期1593-1596,共4页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金;青年科学基金项目(81302890);江苏省高校自然科学基金面上项目(13KJB360003);江苏省自然科学基金面上项目(BK2011816);高等学校博士学科点专项科研基金面上项目(新教师类)(20123237120004);南京中医药大学青年自然科学基金(12XZR09)

摘　　要：目的研究补阳还五汤(黄芪、当归、白芍、地龙、川芎、红花、桃仁)对失神经肌萎缩大鼠Angptl4基因表达的影响,探讨其对失神经肌萎缩的保护机制。方法建立大鼠腓总神经夹伤模型,将造模后的60只SPF级SD大鼠随机分为:假手术组、补阳还五汤高、中、低剂量组、弥可保组与模型组,术后每日灌胃给药。18 d后qRT-PCR与Western-blot检测Angptl4基因与蛋白的差异表达。结果与模型组相比,补阳还五汤中、高剂量组Angptl4 mRNA与蛋白表达均显著增高(P<0.05或P<0.01)。结论补阳还五汤可能通过增加Angptl4基因与蛋白表达,促进血管新生,改善失神经萎缩肌肉的血液供应,从而延缓肌萎缩的发生。AIM To study the effect of Buyang Huanwu Decoction （Astragali Radix, Angelicae sinensis Ra- dix, Paeoniae Radix alba, Pheretima, Chuanxiong Rhizoma, Carthami Flos, Persicae Semen） on Angptl4 and pro- tein gene expression in the denervated tibial muscle atrophy of rats and their protection mechanism. METHODS After 60 Sprague-Dawley rats subjected to common peroneal nerve crush model of 5 mm injury, the rats were ran- domly divided into six groups for daily intragastric administration of drugs ： sham operation group, the model group, high, medium, low dose of Buyang Huanwu Decoction groups, mecobalamin group （as a positive control）. The drug administration lasted for 18 days. Different expression levels of Angptl4 in the tibial muscle of rats after the op- eration were performed by qRT-PCR and Western-blot. RESULTS Compared with the model group, Angptl4 mR- NA and protein expression in Buyang Huanwu Decoction medium and high-dose group increased significantly （P 〈 0. 05 or P 〈0.01 ）. CONCLUSION Buyang Huanwu Decoction maybe increase Angptl4 expression to promote the angiogenesis and improve blood supply for the denervated tibial muscle atrophy of rats.

关 键 词：补阳还五汤 失神经 肌萎缩 大鼠 胫前肌 Angptl4 

分 类 号：R285.5[医药卫生—中药学]