蚕丝蛋白水解液中多肽得率的测定  

Determination of Yield of Peptides in Silk Protein Hydrolysates

作　　者：李琳琳[1] 曹新志[1] 游见明[1] 赵迎庆[1] 

机构地区：[1]四川理工学院生物工程学院,四川自贡643000

出　　处：《食品研究与开发》2014年第13期106-109,共4页Food Research and Development

基　　金：四川省教育厅重点项目(14ZA0207)

摘　　要：用茚三酮显色法测定蚕丝蛋白质水解液中游离氨基酸的含量,15%单宁酸沉淀蛋白质水解液中的大分子蛋白质,经离心过滤后,用凯氏定氮法测定上清液中总蛋白含量,继而测定蚕丝蛋白水解液中多肽的得率。本试验以期用低成本高效率的方法测得多肽的得率,进而可以应用于工业化生产。The content of free amino acid in silk protein hydrolysate is determined by using ninhydrin color reaction. Macromolecule protein in protein hydrolysate is deposited by using 15 %tannic acid. After centrifugalization and filtration, the total content of protein in the clear solution is determined by using Kjeluhl method. Then the yield of peptide in silk protein hydrolysate is obtained. The purpose of this test is to obtain the yield of peptide by using cost-efficient method , then this approach can be applied to industrial production.

关 键 词：茚三酮显色反应 凯氏定氮 单宁酸 多肽得率 

分 类 号：O657.3[理学—分析化学] O629.73[理学—化学]