脂氧合酶OsRCI-1正调控水稻对二化螟的抗性  

Rice lipoxygenase gene OsRCI- 1 mediated pathway positively regulates resistance to Rice Striped Stem Borer

作　　者：姚张良 曹梦娇[1] 王霞[1] 缪家顺[1] 沈励泽[1] 邓建宇[1] 吴慧明[1] 徐志宏[1] 娄永根[2] 周国鑫[1] 

机构地区：[1]浙江农林大学农业与食品科学学院植物保护系浙江省农产品品质改良技术研究重点实验室,浙江临安311300 [2]浙江大学水稻生物学国家重点实验室昆虫科学研究所,浙江杭州310058

出　　处：《环境昆虫学报》2014年第4期507-515,共9页Journal of Environmental Entomology

基　　金：国家自然科学基金(31101451);浙江省自然科学基金(Y3100384);浙江农林大学“青年拨尖人才”和校发展基金(2034070001、2034020061);浙江农林大学研究生科研创新基础项目(3122013240262);2013年浙江省大学生科技创新活动计划项目(2013R412037)

摘　　要：本文克隆了虫害诱导的水稻脂氧合酶基因OsRCI-1全编码序列,实时定量QRT-PCR检测结果表明二化螟Chilo suppressalia Walker取食能快速且持续地诱导OsRCI-1基因的表达。利用农杆菌介导的水稻遗传转化获得了两株OsRCI-1基因的RNAi沉默突变体,突变体株系ir-rci-1和ir-rci-2中目的基因OsRCI-1的表达被明显的抑制,其表达水平分别仅为对照(秀水11)的47.73%和32.33%。生测结果表明OsRCI-1基因沉默导致水稻对二化螟抗性显著地降低,取食RNAi突变体株系的二化螟体重分别是取食秀水11的1.58倍和2.15倍。因此,水稻OsRCI-1基因正调控对二化螟的抗性,该基因介导的防御途径可能在水稻虫害诱导防御反应中起重要的作用。We isolated an herbivore-induced rice lipoxygenase gene OsRCI-1 with full coding sequence （CDS） and its expression levels quickly and constantly increased after rice Striped stem borer （SSB）, Chilo suppressalia Walker treatment Using Agrobacterium-mediated transformation technology, we develop two mutant lines of OsRCI-1 （ir-rci-1 and ir-rci-2）,in which the expression levels of OsRCI-1 were inhibited significantly, the transcript level of OsRCI-1 in ir-rci-1 and -2 was only 4773% and 3233% of level of XS11 Bioassay showed that resistance toward SSB was greatly impaired in mutant rice plants ir-rci lines compared to wild type rice （WT）. The mass of SSB that fed on ir-rci lines was 1.58 and 2.15 times comparing with WT plants, respectively The results indicated that OsRCI-1 gene positively regulated resistance of rice toward rice SSB, which may play an important role in herbivore-indcued defence in rice.

关 键 词：水稻 OsRCI-1 脂氧合酶 茉莉酸 虫害诱导防御 

分 类 号：Q966[生物学—昆虫学] S435.122.1[农业科学—农业昆虫与害虫防治]