理气活血方剂对球囊损伤后实验性大耳白兔低密度脂蛋白胆固醇及血小板源性生长因子B水平的影响  

Impact of Qi and Blood Circulation Prescription on LDL-C and PDGF-B Levels in Experimental Rabbits after Balloon Injury

作　　者：赏楠[1] 

机构地区：[1]辽宁中医药大学附属医院心内VIP科,沈阳110032

出　　处：《医学综述》2014年第15期2810-2812,共3页Medical Recapitulate

摘　　要：目的观察理气活血方剂对球囊损伤后实验性大耳白兔低密度脂蛋白胆固醇(LDL-C)及血小板源性生长因子B(PDGF-B)的影响。方法将32只健康新西兰大耳白兔(雌雄不限)以抽签的方法随机分为正常对照组、模型组、西药组、中药组4组,每组8只,分别给予普通饲料、高脂饲料、高脂饲料加氟伐他汀胶囊、高脂饲料加理气活血方剂以灌胃喂饲。实验第5周,模型组、西药组、中药组均行右侧腹-髂动脉内膜球囊损伤术。第8周给予采血,化验血脂,比较LDL-C的水平。8周后处死所有动物,用反转录-聚合酶链式反应(RT-PCR)方法观察PDGF-B的表达。结果实验动物在第8周实验结束时测定LDL-C,正常组(1.181±0.005)mmol/L,西药组(33.202±7.112)mmol/L,中药组(31.504±9.611)mmol/L,模型组(34.732±6.413)mmol/L,模型组的LDL-C水平高于正常组,中药组和西药组则低于模型组而高于正常组(P<0.05),而中药组和西药组之间的差异无统计学意义;PDGF-B正常组为(0.38±0.09)mg/L,西药组为(1.22±0.09)mg/L,中药组为(1.12±0.07)mg/L,模型组为(1.67±0.16)mg/L,中药组的PDGF-B的表达低于模型组(P<0.05),具有统计学意义。结论理气活血中药方剂可以改善LDL-C的水平,抑制PDGF-B的表达,可以更好地阻断血管平滑肌细胞增殖的关键环节,阻止动脉粥样硬化的发生、发展。Objective To observe the qi and blood circulation prescription on low-density lipoprotein （LDL-C） and platelet-derived growth factor-B（PDGF-B） after balloon injury in experimental rabbits. Methods A total of 32 healthy New Zealand white rabbits （ male or female） were randomly divided into four groups by drawing lots method ： normal control group, model group, Lescol （ fluvastatin ） WM group, Chinese medicine group, were given normal diet, high fat diet, high-fat diet plus Lescol capsules, high-fat diet plus prescription qi and blood circulation respectively. At the end of the fifth week of the experiment, the modal group,WM group and Chinese medicine group, underwent right flank-lilac artery balloon injury intimal surgery. At the eighth week blood was taken, blood lipid was tested, including LDL-C. All animals were sacri- ficed after 8 weeks, RT-PCR （ reverse transcription-polymerase chain reaction） method was used to observe the expression of PDGF-B. Results The LDL-C level at the end of the experiment, the results showed ： normal control group （ 1.181 ± 0. 005 ） mmol/L, Lescol （ fluvastatin ） WM group （ 33.201 ± 7.112 ） mmol/L, Chinese medicine group （ 31. 504 ± 9.611 ） mmol/L, model group （34. 732 ± 6. 413 ） retool/L, LDL-C levels in the model group was higher than the normal group, Chinese medicine and western medicine group was lower than the model group and higher than the normal group （P 〈 0. 05 ）, whereas no significant difference between the Chinese medicine group and western medicine group; RT-PCR results showed PDGF-B level： normal control group （0.38 ± 0.09 ） mg/L, Lescol （ fluvastatin ） WM group （ 1.22 ± 0.09 ） mg/L, Chinese medicine group （ 1.12 ± 0. 07 ） retool/L, model group （ 1.67 ± 0. 16 ） mmol/L. PDGF-C of Chinese medicine group was significantly lower than the model group （ P 〈 0.05 ）. Conclusion Qi and blood cireulatian prescription can improve LDL-C levels, and inhibit the expression of PDGF-B, which can be

关 键 词：理气活血方剂 动脉粥样硬化 血小板源性生长因子-B 低密度脂蛋白胆固醇 

分 类 号：R36[医药卫生—病理学]