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作 者:周占清[1] 赵群芬[1] 陈晓菲[1] 刘林[1]
出 处:《生物学杂志》2014年第4期20-23,共4页Journal of Biology
基 金:宁波大学学科项目(xkl1342);宁波大学SRIP项目
摘 要:研究了纳米ZnO对斑马鱼肝脏生理生化指标的影响。当斑马鱼暴露在ZnO纳米颗粒浓度为2.8 mg/L、5.6mg/L、11.2 mg/L、22.4 mg/L和44.8 mg/L时,分别测定了6 h、12 h、24 h、48 h和72 h时斑马鱼肝组织中的GSH、MDA、Na+K+-ATPase的含量和24 h ROS的变化,结果显示:与对照组相比,处理组中斑马鱼肝组织中GSH的含量显著减少(P<0.05),MDA含量随处理浓度的升高却显著增加(P<0.05),Na+K+-ATPase活性随暴露时间先显著降低后显著升高(P<0.05),24 h ROS含量高于对照组(P<0.05),说明斑马鱼肝组织的生理活动受到ZnO纳米颗粒的影响,且有时间和浓度依赖性。The effects of nano ZnO on the physiological and biochemical index in the liver of zebra fish were studied in this paper. Zebra fish were exposed to the different concentration( 8 mg /L,5. 6 mg /L,11. 2 mg /L,22. 4 mg /L and 44. 8 mg /L) of the nano ZnO.The contents of GSH,MDA,and Na+K+-ATPase in the liver were detected at 6,12,24,48,and 72 hours. Changes of 24 h ROS were also detected. The results showed that the content of GSH in the liver tissues was significantly lower than that in the control group( p 0.05),while the content of MDA in the liver tissues was significantly increased( p 0.05) with the increasing concentration of nano ZnO. The activity of Na+/K+-ATP enzyme in liver tissues was significantly decreased at first,and then increased( p 0. 05)with a longer exposure time. The content of changes of 24 h ROS in the liver was higher than that in the control group( p 0. 05). Experimental results showed that physiology in the liver tissues of zebra fish was affected by the ZnO nanoparticles and also depended on the time and concentration.
分 类 号:X174[环境科学与工程—环境科学]
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