三疣梭子蟹肌肉组织中甜菜碱、糖原及无机盐变化研究  

Research on change of betaine,glycogen and inorganic ions in muscle tissue of Portunus trituberculatus

作　　者：金蕾[1] 徐善良[2] 邱成功[2] 方增冰 

机构地区：[1]宁波大学科学技术学院,浙江宁波315211 [2]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211

出　　处：《生物学杂志》2014年第4期24-28,共5页Journal of Biology

基　　金：国家自然科学基金(31172043);浙江省自然科学基金(LY12C19003)

摘　　要：为了探讨甜菜碱、糖原及无机盐含量对三疣梭子蟹肌肉口感品质的影响,采用可见分光光度法、蒽酮比色法和原子分光光度法,分别测定了3个发育阶段（8月份幼蟹阶段、10月份交配前和12月份交配后）的野生三疣梭子蟹肌肉组织中甜菜碱、糖原及无机盐含量。结果表明：1）肌肉中甜菜碱含量在第一阶段（8月幼蟹）甜菜碱含量最低,为172 mg/100 g,10月交配前雌蟹肌肉甜菜碱含量最高,达473 mg/100 g,但12月份交配后含量减半;雄蟹肌肉则表现为从第1到第3阶段逐渐升高趋势。2）幼蟹的糖原含量极低,仅为0.8μg/mg,交配前雌雄蟹仍保持较低水平,交配后糖原含量急剧升高,雌雄蟹分别上升至11.26μg/mg和9.61μg/mg,各是交配前的6.5倍和10倍。3）肌肉无机盐中K、Na是宏量元素,含量最高,K含量336~457 mg/100 g,Na含量131~185 mg/100 g,Fe为微量元素,含量最低,仅有1.12~1.26 mg/100 g。三疣梭子蟹肌肉中的甜菜碱含量远高于一般水产品,故蟹肉入口爽滑带有甜味,且随着肌肉中糖原含量不断增加,其风味也更佳。In order to explore the effect of betaine,glycogen and inorganic ions on the mouthfeel of Portunus trituberculatus,the betaine,glycogen and inorganic ions of muscle tissue of wild Portunus trituberculatus at three different developmental stages（ juvenile crabs in August,before mating in October and after mating in December） were measured by spectrophotometry,anthrone colorimetric method and atomic spectrophotometry,respectively. The results showed that the contents of betaine in juvenile crabs were the lowest with 172 mg /100 g in August and the highest in female crabs before mating with 473 mg /100 g in October,but after mating,the contents of betaine in female crabs halved; the contents of betaine in the male crabs＇ muscles increased gradually with the growth. The contents of glycogen in juvenile crabs were very low,just 0. 8 μg /mg. The glycogen of male and female crabs kept a lower level before mating,but increased rapidly after mating,the contents of glycogen in male and female crabs rose to 11. 26 μg /mg and 9. 61 μg /mg,respectively.The elements of K and Na were macroelement and were highest in inorganic ions of muscles,the content of K was 336- 457 mg /100 g,and the content of Na was 131- 185 mg /100 g; Fe was microelement,only 1. 12- 1. 26 mg /100 g.

关 键 词：三疣梭子蟹 甜菜碱 糖原 无机盐 营养品质 

分 类 号：S963.161[农业科学—水产养殖]