趋化因子CCL19在结直肠癌中的表达和作用  

Expression of chemokine CCL19 and its role in colorectal carcinoma

作　　者：陆军[1,2] 赵敬坤[1] 陆爱国[1] 冯浩 王蒲雄志[1] 马君俊[1] 宗雅萍[1] 冯波[1] 刘炳亚[2] 郑民华[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院微创外科,上海200025 [2]上海交通大学医学院附属瑞金医院上海市消化外科研究所,上海200025 [3]慕尼黑大学医学院,慕尼黑80539

出　　处：《第二军医大学学报》2014年第7期727-733,共7页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金(81201625);上海市科委医学引导基金(124119a0900)~~

摘　　要：目的观察趋化因子CCL19在结直肠癌组织中的表达,探讨其对结直肠癌细胞的增殖、迁移、侵袭能力的影响。方法收集确诊为结直肠恶性肿瘤并手术切除患者(n=85)的肿瘤组织及癌旁正常组织,运用实时定量PCR和组织微阵列免疫组化技术检测CCL19的表达水平;以实时定量PCR、蛋白质印迹技术筛选高表达CCL19受体CCR7的人结直肠癌细胞株SW620细胞作为研究对象,并给予重组人CCL19(rh-CCL19)刺激,通过细胞增殖实验、划痕实验、Transwell实验来检测SW620细胞的增殖、迁移和侵袭能力的变化。结果结直肠癌组织中的CCL19表达要明显低于癌旁正常组织(P<0.05),且CCL19表达量与肿瘤大小和浸润深度有关(P<0.01)。在CCR7高表达的SW620细胞中,加入rh-CCL19后,其细胞增殖、迁移及侵袭能力下降(P<0.05)。结论 CCL19在结直肠癌组织中的表达量低于癌旁正常组织,且与肿瘤大小和浸润深度有关;CCL19可抑制人结直肠癌细胞株SW620细胞增殖、迁移和侵袭能力,提示CCL19具有抑制结直肠癌的作用。Objective To observe the expression of CCL19 in colorectal carcinoma tissues and to investigate its effect on proliferation,migration and invasion of colorectal cancer cells.Methods The expression of CCL19 in 85 confirmed colorectal carcinoma tissues and the corresponding adjacent normal tissues was detected by quantitative real-time PCR（qRT-PCR）and immunohistochemistry of tissue microarray.SW620 cell line highly expressing CCR7（CCL19 receptor）as screened by qRTPCR and Western blotting analysis was stimulated by different concentrations of rh-CCL19（0,10,and 100ng/mL）.Then the cell proliferation,migration and invasion capacity were examined by CCK-8,wound healing assay,and Transwell assay,respectively.Results Immunohistochemistry and qRT-PCR results demonstrated that CCL19 expression was significantly lower in the colorectal carcinoma tissues than in the normal tissues（P0.05）.CCL19 expression was associated with tumor size and invasion depth（P0.01）.Treatment with rh-CCL19 significantly decreased the proliferation,migration and invasion capacity of SW620cells（P0.05）.Conclusion CCL19 expression in colorectal cancer tissues is lower than that in the adjacent normal tissues,and the expression is associated with tumor size and invasion depth.CCL19 can inhibit the proliferation,migration and invasion capacity of SW620 cells.

关 键 词：结直肠肿瘤 趋化因子CCL19 CCR7受体 细胞增殖 细胞迁移 细胞侵袭 

分 类 号：R735.34[医药卫生—肿瘤]