β-catenin、E-cadherin和Ki67在小鼠大肠癌中的表达及其意义  

Expression of β-catenin, E-cadherin and Ki67 in chemically induced mice colorectal cancer and its significance

作　　者：赵一军[1] 石枕长 韩华中[1] 朱庆超[1] 杨俊[1] 

机构地区：[1]上海交通大学附属第六人民医院普外科,上海200233

出　　处：《中国现代普通外科进展》2014年第6期430-434,共5页Chinese Journal of Current Advances in General Surgery

摘　　要：目的 :探讨小鼠正常肠黏膜组织与大肠癌组织β-catenin、E-cadherin和Ki67表达情况及其关系。方法:采用氧化偶氮甲烷诱导建立小鼠大肠癌模型;运用SP免疫组织化学方法检测20只正常小鼠肠黏膜组织与20只小鼠大肠癌组织中β-catenin、E-cadherin和Ki67的表达情况,并分析三者表达的相关性。结果:β-catenin在小鼠大肠癌组织中的异常表达率(70%)较正常小鼠肠黏膜组织(5%)显著升高(P<0.001);E-cadherin在小鼠大肠癌组织中的阳性率(45%)较正常小鼠肠黏膜组织(95%)显著降低(P<0.001);Ki67在小鼠大肠癌组织中的阳性率(80%)较正常小鼠肠黏膜组织(15%)显著升高(P<0.001)。小鼠大肠癌组织中Ki67的阳性表达与β-catenin的异常表达呈正相关(P<0.001),与E-cadherin的阳性表达呈负相关(P<0.001)。结论:E-cadherin低表达、Ki67高表达以及β-catenin异常表达可能在大肠癌的发生发展和转移过程中发挥着重要作用。Objective：To investigate the relationship of β-catenin,E-cadherin and Ki67 expressions between normal mucosa tissue and colorectal cancer tissues in chemically induced mice.Methods：Azoxymethane was used to induce mice and establish the model of colorectal cancer; SP immunohistochemical method was used to detect β-catenin,E-cadherin and Ki67 expressions in normal mucosa tissue and colorectal cancer tissues in mice.Results：The proportions of abnormal expression of β-catenin were 70％ in colorectal cancer group and 5％ in control group（P＜0.001）.The positive expression rates of E-cadherin were 45％ in colorectal cancer group and 95％ in control group （P＜0.001）.The positive expression rates of Ki67 were 80％ in colorectal cancer group and 15％ in control group （P＜0.001）.Moreover,there was a positive correlation between the β-catenin and Ki67 expressions （P＜0.001）,as well as a negative correlation between the E-cadherin and Ki67 expressions （P＜0.001） in colorectal cancer group.Conclusion：Abnormal expressions of β-catenin,E-cadherin and Ki67 may be associated with the growth and invasion of the colorectal cancer and can be used as a marker of progression for colorectal cancer.

关 键 词：大肠肿瘤 Β-CATENIN E-CADHERIN KI67 小鼠 

分 类 号：R735.34[医药卫生—肿瘤]