HPLC-DAD法测定馒头中胭脂红·诱惑红的含量测定的不确定度评定  

Uncertainty Analysis Determination of Carmine and Temptation in Steamed Bread by High Performance Liquid Chromatography( HPLC) with Diode Array Detection( DAD)

作　　者：王俪瓯 杨微微[1] 胡立立[1] 许乾丽[2] 

机构地区：[1]贵阳医学院,贵州贵阳550001 [2]贵州省食品药品检验所,贵州贵阳550004

出　　处：《安徽农业科学》2014年第24期8367-8369,8390,共4页Journal of Anhui Agricultural Sciences

基　　金：贵州省科技厅课题(黔科合SY字[2011]3090号);黔科平台[2012]4001);国家局餐饮服务食品安全标准制定项目

摘　　要：[目的]采用HPLC-DAD法测定馒头中合成色素胭脂红、诱惑红的含量,分析评定胭脂红、诱惑红的不确定度。[方法]通过HPLC-DAD法测定馒头中胭脂红、诱惑红的含量方法建立数学模型,对各个不确定度因素进行评估、计算,由此计算合成不确定度,最终给出测量结果在95%置信区间下的扩展不确定度。[结果]试验测定的胭脂红含量测定结果为(4.13±0.32)mg/kg;诱惑红含量测定结果为(6.19±0.40)mg/kg。[结论]研究可为食品中色素检测分析应该采取的针对性措施提供参考依据。[Objective] To analyze the uncertainty through the determination of Carmine and Temptation in steamed bread by HPLC with DAD.[Method] The mathematic model was established on determination of Carmine and Temptation by HPLC-DAD.Each component of uncertainty was evaluated,from which the combined uncertainty and finally the extended uncertainty were obtained in 95％ confidence interval.[Result] The determined content of Carmine and Temptation of this test is （4.13 ± 0.32） mg/kg and （6.19 ± 0.40） mg/kg.[Conclusion] The study can provide reference basis for adopting rational countermeasures in pigment detection in food.

关 键 词：HPLC-DAD 馒头 胭脂红 诱惑红 不确定度评定 

分 类 号：S609.9[农业科学—园艺学]