rhGM-CSF和rhG-CSF对A549细胞顺铂化疗敏感性的影响  

The ef ects of rhGM-CSF and rhG-CSF on chemosensitivity of A549 cells to cisplatin

作　　者：冯婷婷[1] 花道金 韩正祥[1] 杜秀平[1] 

机构地区：[1]徐州医学院附属医院肿瘤内科,江苏徐州221000

出　　处：《肿瘤》2014年第7期609-615,共7页Tumor

基　　金：江苏省卫生厅医学科技发展基金会资助项目(编号:P200942)

摘　　要：目的 :研究重组人粒细胞巨噬细胞集落刺激因子(recombinant human granulocyte/macrophage colonystimulating factor,rhGM-CSF)与重组人粒细胞集落刺激因子(recombinant human granulocyte colony-stimulating factor,rhG-CSF)对肺腺癌A549细胞顺铂化疗敏感性的影响。方法 :采用免疫细胞化学法检测A549细胞中GM-CSF及G-CSF受体的表达情况。CCK-8法检测不同浓度rhGM-CSF、rhG-CSF和顺铂对A549细胞增殖的影响,以选择最适浓度进行A549细胞对顺铂联合rhGM-CSF或rhG-CSF化疗敏感性的研究;分别采用CCK-8法、FCM法和Transwell小室法检测rhGM-CSF和rhG-CSF对A549细胞顺铂化疗敏感性的影响。结果 :GMCSF和G-CSF受体在A549细胞上均有表达;rhGM-CSF和rhG-CSF在质量浓度为0.1 ng/mL时促进A549细胞增殖的作用最明显;顺铂在质量浓度2.0μg/mL时对A549细胞的的抑制率为(34.46±2.07)%,4.0μg/mL时抑制率为(65.91±2.78)%,因此选择顺铂浓度为2.0μg/mL,rhGM-CSF和rhG-CSF浓度为0.1 ng/mL进行序贯治疗。顺铂序贯rhGM-CSF组中,与顺铂单药组相比,顺铂联合rhGM-CSF能提高顺铂对A549细胞增殖的抑制作用,但差异无统计学意义;同时能明显增强顺铂诱导A549细胞凋亡,抑制细胞迁移和侵袭的能力,差异均有统计学意义(P<0.05)。而顺铂序贯rhG-CSF组中,与顺铂单药组相比,顺铂联合rhG-CSF明显降低了顺铂抑制A549细胞增殖、诱导细胞凋亡、抑制细胞迁移和侵袭的作用,差异均有统计学意义(P<0.05)。结论 :rhGM-CSF或可增加A549细胞对顺铂的敏感性,而rhG-CSF则可减弱其对顺铂的敏感性。Objective： To investigate the effects of recombinant human granulocyte/macrophage colony-stimulating factor （rhGM-CSF） and recombinant human granulocyte colony-stimulating factor （rhG-CSF） on the chemosensitivity of human lung adenocarcinoma A549 cells to cisplatin. Methods： The expressions of GM-CSF receptor （GM-CSFR） and G-CSF receptor （G-CSFR） in A549 cells were detected by immunocytochemical method. The effects of different concentrations of rhGM-CSF, rhG-CSF and cisplatin on proliferative ability of A549 cells were detected using cell counting kit-8 （CCK-8） to select the most appropriate concentration to examine the effects of rhGM-CSF and rhG-CSF on the chemosensitivity of A549 cells to cisplatin, which were detected by CCK-8, flow cytometry （FCM） and Transwell migration and invasion assays. Results： The GM-CSFR and G-CSFR were expressed in A549 cells. The concentrations of rhGM-CSF and rhG-CSF which could promote the proliferation of A549 cells most effectively both were 0.1 ng/mL. After treatment with 2.0 and 4.0 μg/mL cisplatin, the proliferation inhibitory rates of A549 cells were （34.46 ±2.07）% and （65.91±2.78）%, respectively. Therefore, 2.0 μg/mL cisplatin and 0.1 ng/mL rhGM-CSF or rhG-CSF were used to perform the sequential therapy regimen. As compared with the administration of single cisplatin, the sequential administration of cisplatin and rhGM-CSF could increase the inhibition of cell proliferation induced by cisplatin but with no statistical significance, and this sequential administration could significantly increase the apoptotic rate as well as decrease the migrative and invasive abilities （all P 〈 0.05）. As compared with the administration of single cisplatin,the sequential administration of cisplatin and rhG-CSF could decrease the inhibition of cell proliferation induced by cisplatin with statistical significance （P 〈 0.05）, and this sequential administration could significantly decrease the apoptotic rate as well as increase the migrati

关 键 词：肺肿瘤 药物疗法 联合 顺铂 粒细胞巨噬细胞集落刺激因子 重组 粒细胞集落刺激因子 重组 A549细胞 

分 类 号：R734.2[医药卫生—肿瘤]