猪骨磷蛋白直接盖髓的实验研究  

Experimental study on the effect of the phosphoproteins of pig bone on dental pulp capping

作　　者：万领[1] 李玉晶[2] 葛丽华[2] 宿颖[2] 刘晓勇[3] 

机构地区：[1]首都医科大学口腔医学院综合科,北京100050 [2]首都医科大学口腔医学院口腔医院研究所 [3]首都医科大学口腔医学院病理科

出　　处：《北京口腔医学》2014年第3期141-143,共3页Beijing Journal of Stomatology

摘　　要：目的观察猪骨磷蛋白对修复性牙本质形成的作用。方法用猪骨磷蛋白作盖髓剂,对小型猪健康牙行盖髓术实验,对照采用氢氧化钙盖髓,盖髓2、4、12周后,通过组织病理学观察牙本质桥形成情况。结果猪骨磷蛋白盖髓2周有团块状的修复性牙本质形成,团块周围有整齐排列的方形或矮柱状的类成牙本质细胞;4周可见大量修复性牙本质团块,呈桥形分布,修复性牙本质周围有整齐排列的柱状的类成牙本质细胞,穿髓孔未完全封闭;12周有完整的修复性牙本质桥形成,将穿髓孔封闭,牙本质结构致密,部分为管状牙本质,牙本质桥下方可见排列整齐的的成牙本质细胞。氢氧化钙盖髓2周穿髓孔下方可见薄层坏死组织;4周有修复性牙本质形成,较猪骨磷蛋白盖髓形成的修复性牙本质薄;12周有完整的修复性牙本质桥形成,牙本质结构致密,部分为管状牙本质,其厚度略低于猪骨磷蛋白盖髓。结论猪骨磷蛋白有良好的生物相容性,用其盖髓,能诱导修复性牙本质形成。Objective To investigate the effect of the phosphoproteins of pig bone on dentin mineralization in vivo. Methods The phospboprotein of pig bone was used as pulp capping in the teeth of minipigs and the calcium hydroxide served as control. The minipigs were sacrificed at 14, 30 and 90 days respectively after operation. The teeth were sampled, sliced and stained by HE for histopathological examination. Results The histopathological results revealed that the pulp cells developed into odontoblastoid cells around the major restorative dentin body after two weeks. There was thick restorative dentin body a month later. The bridge was thick and dense and mainly tubular dentin in 3 months. In the calcium hydroxide capping group, inflammatory cytonecrosis zone was found in the surface of dental pulp and only a little calcial bodies formed in two weeks. Complete restorative dentin bridge was formed in 3 mouths. Conclusion The phosphoproteins of Pig Bone can induce pulp cells to develop into odontoblasts and formation of dentin bridge with little stimulation to the dental pulp.

关 键 词：磷蛋白 盖髓 小型猪 

分 类 号：R781.05[医药卫生—口腔医学]