3种拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异  

Behavioral differences in learning and memory abilities among three animal models of Alzheimer's disease assessed by Morris water maze test

作　　者：李斌[1] 谢淑玲[1] 彭丽燕[1] 王飞[1] 杨昆[1] 伍文彬[1] 

机构地区：[1]成都中医药大学临床医学院,成都医学博士610075

出　　处：《医学研究生学报》2014年第7期683-685,共3页Journal of Medical Postgraduates

基　　金：国家自然科学基金(81303283);四川省科技创新苗子工程资助项目(20132054)

摘　　要：目的 Morris水迷宫实验是一种评价空间学习记忆能力的经典方法,是评价痴呆动物模型复制结果的客观指标。近年来痴呆动物模型已成为研究老年性痴呆的重要手段。文中评价3种常见拟痴呆动物模型在Morris水迷宫行为学测试中学习记忆行为的差异。方法分别使用SD大鼠、昆明种小鼠以及快速老化小鼠制备Aβ脑室注射致痴呆模型、东莨菪碱腹腔注射致记忆障碍模型、快速老化模型。采用Morris水迷宫行为学测试方法,将这3种模型通过与其相应的对照组比较,考察其行为学差异。结果东莨菪碱模型与快速老化模型逃避潜伏期时间短于相应对照组,其中以东莨菪碱模型差异尤为显著[(35.47±3.78)s vs(50.61±3.94)s,P<0.01];快速老化组模型与对照组差异有统计学意义[(17.39±4.71)s vs(33.79±7.81)s,P<0.05];Aβ致痴呆模型与其对照组比较,模型定位航行实验成绩差异无统计学意义(P>0.05)。结论东莨菪碱腹腔注射致记忆障碍模型较其他2种常用模型更为理想。Morris水迷宫行为学测试方法是一种评价拟痴呆动物的理想方法。Objective The study was to evaluate the behavioral differences in learning and memory abilities among three ani -mal models of Alzheimer′s disease. Methods Three SD rat models（n=20） were used in this study.The first SD rat model was es-tablished by intracerebral injection of Aβ25-35 into the bilateral hippocampus , the second mouse model was induced by intraperitoneal in-jection of scopolamine and the third was a senescence accelerated mouse model .Morris Water maze test was performed to investigate behavioral differences by comparison to corresponding blank control groups （ sham operation group , blank group and P8 group ） . Results The learning and memory abilities of senescence accelerated rats and scopolamine -treated rats were worse than those of the corresponding control groups, especially the scopolamine-treated rat （[35.47 ±3.78]s vs [50.61 ±3.94]s, P〈0.01）.There was no significant difference between Aβrats and sham-operated rats（P〉0.05）. Conclusion The model of Alzheimer′s disease in-duced by intraperitoneal injection of scopolamine represents more distinct changes in learning and memory abilities .Morris water maze test can be used to well evaluate whether the scopolamine-induced model is successfully established or not .

关 键 词：拟痴呆模型 Morris水迷宫试验 行为学差异 认知与记忆能力 大鼠 小鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]